{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T17:49:44Z","timestamp":1770486584588,"version":"3.49.0"},"reference-count":18,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,5,4]],"date-time":"2016-05-04T00:00:00Z","timestamp":1462320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Italian Ministry of Health","award":["44\/GR-2010-2300447"],"award-info":[{"award-number":["44\/GR-2010-2300447"]}]},{"name":"Fondazione Benefica Alberto & Kathleen Casali"},{"name":"Fondazione Beneficentia Stiftung"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>This study evaluates the association between the long noncoding <jats:styled-content style=\"fixed-case\">RNA GAS<\/jats:styled-content>5 levels and the anti\u2010proliferative effect of the glucocorticoid (<jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>) methylprednisolone (<jats:styled-content style=\"fixed-case\">MP<\/jats:styled-content>) alone and in combination with rapamycin in peripheral blood mononuclear cells (<jats:styled-content style=\"fixed-case\">PBMC<\/jats:styled-content>s) obtained from healthy donors. The effect of <jats:styled-content style=\"fixed-case\">MP<\/jats:styled-content>, rapamycin, and <jats:styled-content style=\"fixed-case\">MP<\/jats:styled-content> plus rapamycin was determined in 17 healthy donors by labelling metabolically active cells with [methyl\u20103H] thymidine and the expression levels of <jats:styled-content style=\"fixed-case\">GAS<\/jats:styled-content>5 gene were evaluated by real\u2010time <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> TaqMan analysis. We confirmed a role for <jats:styled-content style=\"fixed-case\">GAS<\/jats:styled-content>5 in modulating <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> response: poor responders presented higher levels of <jats:styled-content style=\"fixed-case\">GAS<\/jats:styled-content>5 in comparison with good responders. Interestingly, when <jats:styled-content style=\"fixed-case\">PBMC<\/jats:styled-content>s were treated with the combination of rapamycin plus <jats:styled-content style=\"fixed-case\">MP<\/jats:styled-content>, the high levels of <jats:styled-content style=\"fixed-case\">GAS<\/jats:styled-content>5 observed for each drug in the <jats:styled-content style=\"fixed-case\">MP<\/jats:styled-content> poor responders group decreased in comparison with rapamycin (<jats:italic>P<\/jats:italic> value\u00a0=\u00a00.0134) or <jats:styled-content style=\"fixed-case\">MP<\/jats:styled-content> alone (<jats:italic>P<\/jats:italic> value\u00a0=\u00a00.0193). <jats:styled-content style=\"fixed-case\">GAS<\/jats:styled-content>5 is involved in <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> resistance and co\u2010treatment of rapamycin with <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>s restores <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> effectiveness in poor responders through the downregulation of the long noncoding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">GAS<\/jats:styled-content>5 could be considered a biomarker to personalize therapy and a novel therapeutic target useful for the development of new pharmacological approaches to restore <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> sensitivity.<\/jats:p>","DOI":"10.1111\/1440-1681.12572","type":"journal-article","created":{"date-parts":[[2016,3,22]],"date-time":"2016-03-22T13:06:07Z","timestamp":1458651967000},"page":"602-605","source":"Crossref","is-referenced-by-count":17,"title":["Differential expression of <scp>GAS<\/scp>5 in rapamycin\u2010induced reversion of glucocorticoid resistance"],"prefix":"10.1111","volume":"43","author":[{"given":"Marianna","family":"Lucaf\u00f2","sequence":"first","affiliation":[{"name":"Department of Medicine, Surgery and Health Sciences University of Trieste Trieste Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa","family":"Bravin","sequence":"additional","affiliation":[{"name":"Department of Life Sciences University of Trieste Trieste Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Tommasini","sequence":"additional","affiliation":[{"name":"Institute for Maternal and Child Health IRCCS \u201cBurlo Garofolo\u201d Trieste Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Martelossi","sequence":"additional","affiliation":[{"name":"Institute for Maternal and Child Health IRCCS \u201cBurlo Garofolo\u201d Trieste Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid","family":"Rabach","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgery and Health Sciences University of Trieste Trieste Italy"},{"name":"Institute for Maternal and Child Health IRCCS \u201cBurlo Garofolo\u201d Trieste Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Ventura","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgery and Health Sciences University of Trieste Trieste Italy"},{"name":"Institute for Maternal and Child Health IRCCS \u201cBurlo Garofolo\u201d Trieste Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuliana","family":"Decorti","sequence":"additional","affiliation":[{"name":"Department of Life Sciences University of Trieste Trieste Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"De Iudicibus","sequence":"additional","affiliation":[{"name":"Institute for Maternal and Child Health IRCCS \u201cBurlo Garofolo\u201d Trieste Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(93)90184-L"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70114-5"},{"key":"e_1_2_7_4_1","first-page":"263","article-title":"Immunosuppressive therapy for autoimmune diseases","volume":"70","author":"Hoffman GS","year":"1993","journal-title":"Ann. 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