{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T16:24:36Z","timestamp":1776183876376,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,7,4]],"date-time":"2016-07-04T00:00:00Z","timestamp":1467590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Strategic Research Foundation at Private Universities","award":["S1411019"],"award-info":[{"award-number":["S1411019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>5\u2010fluorouracil (5\u2010<jats:styled-content style=\"fixed-case\">FU<\/jats:styled-content>) is part of the standard care for cancer treatment but is associated with high incidences of appetite loss and reduced food intake, which may contribute to chemotherapy\u2010induced cachexia (weakness and wasting of tissue). The role of gastrointestinal satiety hormones in chemotherapy\u2010induced appetite loss has not been intensively investigated. Peptide<jats:styled-content style=\"fixed-case\">YY<\/jats:styled-content>(<jats:styled-content style=\"fixed-case\">PYY<\/jats:styled-content>) and glucagon\u2010like peptide (<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>)\u20101 are important signals of gastrointestinal satiety, so this study examined the roles of these gut hormones in 5\u2010<jats:styled-content style=\"fixed-case\">FU<\/jats:styled-content>\u2010induced reduction of dietary intake. Mice were given 5\u2010<jats:styled-content style=\"fixed-case\">FU<\/jats:styled-content>(50\u00a0mg\/kg, intraperitoneal [i.p.]) every day for 4 consecutive days. Gene expression levels of proglucagon (Pro\u2010Gcg), a precursor of<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20101, and<jats:styled-content style=\"fixed-case\">PYY<\/jats:styled-content>in the colon were examined by real\u2010time<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Serum levels of<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20101 and<jats:styled-content style=\"fixed-case\">PYY<\/jats:styled-content>were measured by enzyme\u2010linked immunosorbent assay. Some mice were pretreated with the<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20101 receptor antagonist exendin9\u201339 (1\u00a0mg\/kg) or the neuropeptide Y type 2 (<jats:styled-content style=\"fixed-case\">NPY<\/jats:styled-content>2) receptor antagonist<jats:styled-content style=\"fixed-case\">BIIE<\/jats:styled-content>0246 (2\u00a0mg\/kg)<jats:italic>via<\/jats:italic>the i.p. route 30\u00a0minutes before 5\u2010<jats:styled-content style=\"fixed-case\">FU<\/jats:styled-content>administration. Mice receiving 5\u2010<jats:styled-content style=\"fixed-case\">FU<\/jats:styled-content>exhibited a significant reduction in food intake that was correlated with body weight loss. These mice also showed significantly enhanced expression levels of<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s encoding pro\u2010<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20101 and<jats:styled-content style=\"fixed-case\">PYY<\/jats:styled-content>in the transverse and distal colon as well as elevated serum concentrations of<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20101 and<jats:styled-content style=\"fixed-case\">PYY<\/jats:styled-content>compared to vehicle\u2010treated controls. The 5\u2010<jats:styled-content style=\"fixed-case\">FU<\/jats:styled-content>\u2010induced reduction in food intake was attenuated by<jats:styled-content style=\"fixed-case\">BIIE<\/jats:styled-content>0246 but not by exendin9\u201339. These data suggest that administration of a<jats:styled-content style=\"fixed-case\">NPY<\/jats:styled-content>2 receptor antagonist may be effective for attenuating the anorexia caused by 5\u2010<jats:styled-content style=\"fixed-case\">FU<\/jats:styled-content>chemotherapy.<\/jats:p>","DOI":"10.1111\/1440-1681.12588","type":"journal-article","created":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T11:16:15Z","timestamp":1462101375000},"page":"753-759","source":"Crossref","is-referenced-by-count":8,"title":["Role of peptide<scp>YY<\/scp>in 5\u2010fluorouracil\u2010induced reduction of dietary intake"],"prefix":"10.1111","volume":"43","author":[{"given":"Hiroyasu","family":"Sakai","sequence":"first","affiliation":[{"name":"Division of Pharmacy Professional Development and Research Department of Analytical Pathophysiology Hoshi University Shinagawa\u2010ku Tokyo 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