{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T18:34:09Z","timestamp":1774463649478,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,11,21]],"date-time":"2016-11-21T00:00:00Z","timestamp":1479686400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["14\u201004\u201031258"],"award-info":[{"award-number":["14\u201004\u201031258"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["#0324\u20102015\u20100004"],"award-info":[{"award-number":["#0324\u20102015\u20100004"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2016,12]]},"abstract":"Summary<\/jats:title>Vasopressin (AVP<\/jats:styled-content>) regulates the body salt\u2010water balance. Brattleboro rats carry an AVP<\/jats:styled-content> gene mutation resulting in a recessive form of central diabetes insipidus, being ideal for AVP<\/jats:styled-content> deficiency studies. Herein, we studied the water permeability of the apical and basolateral sides of outer medullary collecting duct (OMCD<\/jats:styled-content>) principal cells in response to dDAVP<\/jats:styled-content> (a V2 receptor agonist) administration in Wistar and Brattleboro rats. Biophysical measurements of the water permeability (P<\/jats:italic>f<\/jats:sub>) of isolated OMCD<\/jats:styled-content> principal cells were performed with the calcein quenching method with\/without dDAVP<\/jats:styled-content> (10\u22128<\/jats:sup>\u00a0mol\/L). mRNA<\/jats:styled-content> transcripts and protein levels of AQP<\/jats:styled-content>2, AQP<\/jats:styled-content>3 and AQP<\/jats:styled-content>4 were assessed by RT<\/jats:styled-content>\u2010PCR<\/jats:styled-content> and western blot respectively. dDAVP<\/jats:styled-content> increased the apical and basolateral P<\/jats:italic>f<\/jats:sub> of OMCD<\/jats:styled-content> principal cells in Wistar rats, while in Brattleboro rats this effect was present basolaterally. Long\u2010term dDAVP<\/jats:styled-content> administration in both strains resulted in a significant increase in mRNA<\/jats:styled-content> expression of all assessed AQP<\/jats:styled-content>'s while only the protein levels of AQP<\/jats:styled-content>2 and AQP<\/jats:styled-content>3 were significantly increased. Short\u2010term (20\u00a0minutes) dDAVP<\/jats:styled-content> treatment of isolated OMCD<\/jats:styled-content> fragments resulted in significantly increased plasma membrane expression of AQP<\/jats:styled-content>2 in Wistar rats and of AQP<\/jats:styled-content>2 and AQP<\/jats:styled-content>3 in Brattleboro rats. In summary, dDAVP<\/jats:styled-content> induces different expression of AQP<\/jats:styled-content>2, AQP<\/jats:styled-content>3 and AQP<\/jats:styled-content>4 in Wistar and Brattleboro rats during short\u2010 and long\u2010term treatment. In Wistar rats dDAVP<\/jats:styled-content> mainly increased AQP<\/jats:styled-content>2 expression while in Brattleboro rats it increased functional water permeability mainly by AQP3 expression.<\/jats:p>","DOI":"10.1111\/1440-1681.12666","type":"journal-article","created":{"date-parts":[[2016,9,5]],"date-time":"2016-09-05T03:22:32Z","timestamp":1473045752000},"page":"1225-1233","source":"Crossref","is-referenced-by-count":5,"title":["Brattleboro rats have impaired apical membrane water permeability regulation in the outer medullary collecting duct principal cells"],"prefix":"10.1111","volume":"43","author":[{"given":"Galina S","family":"Baturina","sequence":"first","affiliation":[{"name":"Institute of Cytology and Genetics, SB RAS Novosibirsk Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liubov E","family":"Katkova","sequence":"additional","affiliation":[{"name":"Institute of Cytology and Genetics, SB RAS Novosibirsk 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