{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:36:33Z","timestamp":1790627793360,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,2,14]],"date-time":"2017-02-14T00:00:00Z","timestamp":1487030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81300202"],"award-info":[{"award-number":["81300202"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Oxidative stress plays an important role in atherosclerosis, a vascular disease with high morbidity and mortality. The <jats:styled-content style=\"fixed-case\">ETS<\/jats:styled-content> domain\u2010containing protein <jats:styled-content style=\"fixed-case\">ELK<\/jats:styled-content>1 is an oxidative stress\u2010sensitive factor modulated by the extracellular signal\u2010regulated kinase (<jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>) 1\/2 pathway. However, the role of <jats:styled-content style=\"fixed-case\">ELK<\/jats:styled-content>1 in the prevention of atherosclerosis by pravastatin remains unclear. In the present study, male apolipoprotein E\u2010knockout (apoE<jats:sup>\u2212\/\u2212<\/jats:sup>) mice fed a diet containing 1.25% cholesterol (w\/w) were divided into two groups, one treated with pravastatin (80\u00a0mg\/kg, 2\u20102.4\u00a0mg\/mouse per day) for 8\u00a0weeks and the other not. Male C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6J mice fed with a normal diet were used as a control group. Human umbilical vein endothelial cells (<jats:styled-content style=\"fixed-case\">HUVEC<\/jats:styled-content>) were cultured and treated with pravastatin (10\u00a0\u03bcmol\/L) for 18\u00a0hours before testing for the presence or absence of 100\u00a0\u03bcmol\/L H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> (24\u00a0hours). Examination of pathological sections from mice aortas revealed that pravastatin treatment almost prevented atherosclerotic plaque formation. Pravastatin also inhibited increases in serum and aortic levels of oxidized low\u2010density lipoprotein and aortic malondialdehyde levels and decreases in aortic reduced glutathione, and the activities of superoxide dismutase, catalase and glutathione peroxidase. H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced increases in reactive oxygen species in <jats:styled-content style=\"fixed-case\">HUVEC<\/jats:styled-content>s were reversed by pravastatin by 48%. Pravastatin blocked the phosphorylation of <jats:styled-content style=\"fixed-case\">ELK<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>1\/2 proteins and reduced <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels of early growth response 1, a known atherogenic transcription factor upregulated by the <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">ELK<\/jats:styled-content>1 pathway, in mice. In conclusion, pravastatin attenuates the action of <jats:styled-content style=\"fixed-case\">ELK<\/jats:styled-content>1 induced by oxidative stress to prevent atherosclerosis, which is dependent partly on modulation of <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>1\/2 signalling.<\/jats:p>","DOI":"10.1111\/1440-1681.12710","type":"journal-article","created":{"date-parts":[[2016,12,20]],"date-time":"2016-12-20T22:52:38Z","timestamp":1482274358000},"page":"344-352","source":"Crossref","is-referenced-by-count":9,"title":["Pravastatin attenuates the action of the ETS domain\u2010containing protein ELK1 to prevent atherosclerosis in apolipoprotein E\u2010knockout mice via modulation of extracellular signal\u2010regulated kinase 1\/2 signal pathway"],"prefix":"10.1111","volume":"44","author":[{"given":"Wei","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Cardiology The First Affiliated Hospital of Harbin Medical University  Harbin 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