{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T12:02:20Z","timestamp":1784116940800,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,4,21]],"date-time":"2017-04-21T00:00:00Z","timestamp":1492732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2016J01506"],"award-info":[{"award-number":["2016J01506"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Ropivacaine is one of the most common but toxic local anaesthetics, and the mechanisms underlying its neurotoxicity are still largely unknown. This study was conducted to prepare a ropivacaine\u2010induced neuronal injury model and research the effects of ropivacaine on <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>\u20101 activation and nicotinamide adenine dinucleotide (<jats:styled-content style=\"fixed-case\">NAD<\/jats:styled-content>)<jats:sup>+<\/jats:sup> depletion. The cell death and apoptosis of ropivacaine\u2010induced <jats:styled-content style=\"fixed-case\">SH<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">SY<\/jats:styled-content>5Y cells were detected with flow cytometry. The lactate dehydrogenase cycling reaction measured the <jats:styled-content style=\"fixed-case\">NAD<\/jats:styled-content><jats:sup>+<\/jats:sup> level, and western blots were used to analyze the expression levels of <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>\u20101 and apoptosis\u2010inducing factor (<jats:styled-content style=\"fixed-case\">AIF<\/jats:styled-content>) after ropivacaine treatments with different concentrations and durations. A <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>\u20101 inhibitor (<jats:styled-content style=\"fixed-case\">PJ<\/jats:styled-content>\u201034) was used to confirm the relationship between <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>\u20101 activation and <jats:styled-content style=\"fixed-case\">NAD<\/jats:styled-content><jats:sup>+<\/jats:sup> depletion. Hoechst 33258 nuclear staining and a mitochondrial membrane potential (\u0394\u03c8m) assay were used to detect the role of exogenous <jats:styled-content style=\"fixed-case\">NAD<\/jats:styled-content><jats:sup>+<\/jats:sup> in ropivacaine\u2010induced neuronal injury. Ropivacaine\u2010induced <jats:styled-content style=\"fixed-case\">SH<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">SY<\/jats:styled-content>5Y cell death and apoptosis, <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>\u20101 activation, and <jats:styled-content style=\"fixed-case\">AIF<\/jats:styled-content> increase as well as intracellular <jats:styled-content style=\"fixed-case\">NAD<\/jats:styled-content><jats:sup>+<\/jats:sup> depletion occurred in a time\u2010 and concentration\u2010dependent manner (<jats:italic>P<\/jats:italic>&lt;.05). <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>\u20101 activation led to <jats:styled-content style=\"fixed-case\">NAD<\/jats:styled-content><jats:sup>+<\/jats:sup> depletion (<jats:italic>P<\/jats:italic>&lt;.05). Exogenous <jats:styled-content style=\"fixed-case\">NAD<\/jats:styled-content><jats:sup>+<\/jats:sup> impaired ropivacaine\u2010induced nuclear injury (<jats:italic>P<\/jats:italic>&lt;.05). Ropivacaine treatment induced <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>\u20101 activation and <jats:styled-content style=\"fixed-case\">NAD<\/jats:styled-content><jats:sup>+<\/jats:sup> depletion (<jats:italic>P<\/jats:italic>&lt;.05). Parthanatos (<jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>\u20101\u2010dependent cell death) was definitely involved in ropivacaine\u2010induced neuronal injury, and exogenous <jats:styled-content style=\"fixed-case\">NAD<\/jats:styled-content><jats:sup>+<\/jats:sup> may be a novel therapeutic method for parthanatos\u2010dependent neuronal injury.<\/jats:p>","DOI":"10.1111\/1440-1681.12730","type":"journal-article","created":{"date-parts":[[2017,1,13]],"date-time":"2017-01-13T01:56:23Z","timestamp":1484272583000},"page":"586-594","source":"Crossref","is-referenced-by-count":13,"title":["Effect of parthanatos on ropivacaine\u2010induced damage in SH\u2010SY5Y cells"],"prefix":"10.1111","volume":"44","author":[{"given":"Ting","family":"Zheng","sequence":"first","affiliation":[{"name":"Fujian Provincial Clinical College Medical College Fujian Medical University  Fuzhou China"},{"name":"Department of Anaesthesiology Fujian Provincial Hospital Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010ying","family":"Zheng","sequence":"additional","affiliation":[{"name":"Fujian Provincial Clinical College Medical College Fujian Medical University  Fuzhou China"},{"name":"Department of Anaesthesiology Fujian Provincial Hospital Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010chun","family":"Zheng","sequence":"additional","affiliation":[{"name":"Fujian Provincial Clinical College Medical College Fujian Medical University  Fuzhou China"},{"name":"Department of Anaesthesiology Fujian Provincial Hospital Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruo\u2010guang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Fujian Provincial Clinical College Medical College Fujian Medical University  Fuzhou China"},{"name":"Department of Anaesthesiology Fujian Provincial Hospital Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7220-9338","authenticated-orcid":false,"given":"Yan\u2010qing","family":"Chen","sequence":"additional","affiliation":[{"name":"Fujian Provincial Clinical College Medical College Fujian Medical University  Fuzhou China"},{"name":"Department of Anaesthesiology Fujian Provincial Hospital Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/159864"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/78.5.507"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1213\/ane.0b013e31819385e1"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2011.01.055"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.7.1001"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2741\/3297"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuro.2005.01.012"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1427.028"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.2741\/2267"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"18314","DOI":"10.1073\/pnas.0606528103","article-title":"Apoptosis 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