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We have previously found that hydrogen sulphide (H<jats:sub>2<\/jats:sub>S), the third endogenous gaseous mediator, protects neuron against the toxicity of <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content>. However, the underlying mechanism is poor. Aldehyde\u2010dehydrogenase\u20102 (<jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content>2) plays a major role in detoxification of reactive aldehyde in a range of organs and cell types. Therefore, we speculated that H<jats:sub>2<\/jats:sub>S antagonizes <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content>\u2010induced neurotoxicity by modulating <jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content>2. In the present study, we found that the exposure of <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>12 cells to <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content> causes increase in <jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content>2 expression and activity. Daidzin, an inhibitor of <jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content>2, significantly antagonizes <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content>\u2010exerted cytotoxicity and oxidative stress including the accumulation of intracellular reactive oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>), 4\u2010hydroxy\u20102\u2010trans\u2010nonenal (4\u2010<jats:styled-content style=\"fixed-case\">HNE<\/jats:styled-content>), and malondialdehyde (<jats:styled-content style=\"fixed-case\">MDA<\/jats:styled-content>), in PC12 cells. We also showed that daidzin markedly attenuated <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content>\u2010induced apoptosis in PC12 cells. Furthermore, we found that H<jats:sub>2<\/jats:sub>S reverses <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content>\u2010elicited upregulation of <jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content>2 in <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>12 cells. Our results demonstrated the involvement of downregulation of <jats:styled-content style=\"fixed-case\">ALDH<\/jats:styled-content>2 in the protection of H<jats:sub>2<\/jats:sub>S against <jats:styled-content style=\"fixed-case\">FA<\/jats:styled-content> neurotoxicity.<\/jats:p>","DOI":"10.1111\/1440-1681.12741","type":"journal-article","created":{"date-parts":[[2017,3,2]],"date-time":"2017-03-02T08:16:56Z","timestamp":1488442616000},"page":"595-601","source":"Crossref","is-referenced-by-count":14,"title":["Inhibition of <scp>ALDH<\/scp>2 protects <scp>PC<\/scp>12 cells against formaldehyde\u2010induced cytotoxicity: involving the protection of hydrogen sulphide"],"prefix":"10.1111","volume":"44","author":[{"given":"Ying","family":"Chen","sequence":"first","affiliation":[{"name":"Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study Institute of Pharmacy and Pharmacology University of South China  Hengyang Hunan China"},{"name":"Institute of Neuroscience 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