{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T22:44:36Z","timestamp":1778885076933,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,7,17]],"date-time":"2017-07-17T00:00:00Z","timestamp":1500249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Guangxi Scientific Research and Technological Development Projects","award":["GKG1355005-4-3"],"award-info":[{"award-number":["GKG1355005-4-3"]}]},{"name":"Youth Fund Project of the Guangxi Natural Science Foundation","award":["2013GXNSFBA019153"],"award-info":[{"award-number":["2013GXNSFBA019153"]}]},{"name":"Guangxi Scientific and Technological Research Projects of Universities","award":["KY2015ZD062"],"award-info":[{"award-number":["KY2015ZD062"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Apoptosis and oxidative stress are considered to be the major factors associated with the development and progression of many ischaemic cerebrovascular diseases. Naringenin (<jats:styled-content style=\"fixed-case\">NAR<\/jats:styled-content>) is an abundant flavanone in citrus plants and has been found to exhibit anti\u2010oxidant, anti\u2010carcinogenic and anti\u2010apoptotic effects. This study aimed to investigate the anti\u2010apoptotic and anti\u2010oxidant effects of naringenin on ischaemic stroke. In vitro, cortical neuron cells isolated from the brains of neonatal Sprague\u2013Dawley rats were randomly divided into control, oxygen and glucose deprivation\/reperfusion (<jats:styled-content style=\"fixed-case\">OGD<\/jats:styled-content>\/Rep), <jats:styled-content style=\"fixed-case\">NAR<\/jats:styled-content>\u2010L, <jats:styled-content style=\"fixed-case\">NAR<\/jats:styled-content>\u2010M and <jats:styled-content style=\"fixed-case\">NAR<\/jats:styled-content>\u2010H groups. <jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> were used for cell proliferation and apoptosis\u2010related proteins analyses. The effects of <jats:styled-content style=\"fixed-case\">NAR<\/jats:styled-content> on the Nrf2 signalling pathway were investigated using transfection approaches. Differences in mitochondrial dysfunction were analyzed by flow cytometry. In vivo, middle cerebral artery occlusion (<jats:styled-content style=\"fixed-case\">MCAO<\/jats:styled-content>) model was prepared and neurological defects and the brain wet\/dry (W\/D) ratio were assessed and recorded; apoptosis was measured based on the <jats:styled-content style=\"fixed-case\">TUNEL<\/jats:styled-content> assay. Additionally, biochemical indices were detected both in vitro and in vivo. <jats:styled-content style=\"fixed-case\">NAR<\/jats:styled-content> promoted cortical neuron cell proliferation, inhibited apoptosis and oxidative stress, and regulated the localization of Nrf2 protein (<jats:italic>P<\/jats:italic>&lt;.05). Furthermore, silencing and overexpression of Nrf2 affected cortical neuron cell proliferation and apoptosis (<jats:italic>P<\/jats:italic>&lt;.05). In vivo, <jats:styled-content style=\"fixed-case\">NAR<\/jats:styled-content> could alleviate cerebral oedema, improve neurological defects, and reduce apoptosis and oxidative stress (<jats:italic>P<\/jats:italic>&lt;.05). These findings demonstrated that <jats:styled-content style=\"fixed-case\">NAR<\/jats:styled-content> could reduce apoptosis and oxidative stress and that Nrf2 signalling pathway is involved in this regulatory process. <jats:styled-content style=\"fixed-case\">NAR<\/jats:styled-content> has health\u2010promoting properties because of its anti\u2010apoptotic and anti\u2010oxidant effects in cases of ischaemic stroke.<\/jats:p>","DOI":"10.1111\/1440-1681.12775","type":"journal-article","created":{"date-parts":[[2017,4,28]],"date-time":"2017-04-28T14:29:42Z","timestamp":1493389782000},"page":"862-871","source":"Crossref","is-referenced-by-count":77,"title":["Naringenin prevents ischaemic stroke damage via anti\u2010apoptotic and anti\u2010oxidant effects"],"prefix":"10.1111","volume":"44","author":[{"given":"Kaihua","family":"Wang","sequence":"first","affiliation":[{"name":"Southern Medical University  Guangzhou China"},{"name":"Department of Neurology RuiKang Hospital Affiliated to Guangxi University of Chinese Medicine  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenzhen","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangxi University of Chinese Medicine  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianmin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Neurology RuiKang Hospital Affiliated to Guangxi University of Chinese Medicine  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Longjian","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Neurology RuiKang Hospital Affiliated to Guangxi University of Chinese Medicine  Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Neurology RuiKang Hospital Affiliated to Guangxi University of Chinese Medicine  Nanning 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