{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T01:13:29Z","timestamp":1768439609065,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,9,5]],"date-time":"2017-09-05T00:00:00Z","timestamp":1504569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004508","name":"Prince of Songkla University","doi-asserted-by":"publisher","award":["SCI580768S"],"award-info":[{"award-number":["SCI580768S"]}],"id":[{"id":"10.13039\/501100004508","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The loop diuretic furosemide has an action to inhibit Na<jats:sup>+<\/jats:sup>\u2013K<jats:sup>+<\/jats:sup>\u20132Cl<jats:sup>\u2212<\/jats:sup>co\u2010transporter at the thick ascending limb of Henle's loop resulting in diuresis. Furosemide also has the non\u2010diuretic effects by binding to<jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content>\u2010A receptor which may involve the gastrointestinal tract. The aim of this study was to investigate the effects of furosemide on smooth muscle contractions in mice ileum and proximal colon. Each intestinal segment suspended in an organ bath was connected to a force transducer. Signal output of mechanical activity was amplified and recorded for analysis using PowerLab System. After equilibration, the intestine was directly exposed to furosemide,<jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content>\u2010A receptor agonist (muscimol), or muscarinic receptor antagonist (atropine). Furosemide (50, 100 and 500\u00a0\u03bcmol\u00a0L<jats:sup>\u22121<\/jats:sup>) acutely reduced the amplitude of ileal and colonic contraction. In the ileum, 1\u00a0mmol\u00a0L<jats:sup>\u22121<\/jats:sup><jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content>and 10\u201060\u00a0\u03bcmol\u00a0L<jats:sup>\u22121<\/jats:sup>muscimol significantly increased the amplitude, whereas in the colon, 50\u2010100\u00a0mmol\u00a0L<jats:sup>\u22121<\/jats:sup><jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content>and 60\u00a0\u03bcmol\u00a0L<jats:sup>\u22121<\/jats:sup>muscimol decreased the contractions. The contractions were also significantly suppressed by atropine. To investigate the mechanisms underlying the inhibiting effect of furosemide, furosemide was added to the organ bath prior to the addition of muscimol or atropine. A comparison of furosemide combined with muscimol or atropine group and furosemide group showed no significant difference of the ileal contraction, but the amplitude of colonic contraction significantly decreased when compared to adding furosemide alone. These results suggest that furosemide can reduce the ileal and proximal colonic contraction mediated by blocking and supporting of<jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content>\u2010A receptor, respectively, resulting in decreased acetylcholine release.<\/jats:p>","DOI":"10.1111\/1440-1681.12824","type":"journal-article","created":{"date-parts":[[2017,7,26]],"date-time":"2017-07-26T08:36:34Z","timestamp":1501058194000},"page":"1155-1165","source":"Crossref","is-referenced-by-count":6,"title":["Furosemide suppresses ileal and colonic contractility via interactions with<scp>GABA<\/scp>\u2010A receptor in mice"],"prefix":"10.1111","volume":"44","author":[{"given":"Kannaree","family":"Kaewsaro","sequence":"first","affiliation":[{"name":"Department of Physiology Faculty of Science Prince of Songkla University Hat Yai Songkhla Thailand"},{"name":"Department of Biology, Faculty of Science Prince of Songkla University Hat Yai Songkhla 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