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A model of contact dermatitis involving repeated challenge of a hapten is adapted to assess dermatitis as characterized by skin thickening. Recently, it was reported that neutrophils have crucial roles in contact hypersensitivity. We thus examined the involvement of<jats:styled-content style=\"fixed-case\">CXC<\/jats:styled-content>chemokines bearing the glutamic acid\u2013leucine\u2013arginine (<jats:styled-content style=\"fixed-case\">ELR<\/jats:styled-content>) motif (\u201c<jats:styled-content style=\"fixed-case\">ELR<\/jats:styled-content><jats:sup>+<\/jats:sup>chemokines\u201d) and neutrophils in the ear swelling induced by 2,4,6\u2010trinitrochlorobenzene (<jats:styled-content style=\"fixed-case\">TNCB<\/jats:styled-content>) challenges in the present study. Mice were sensitized by application of<jats:styled-content style=\"fixed-case\">TNCB<\/jats:styled-content>on their abdominal skin. They were then challenged thrice with<jats:styled-content style=\"fixed-case\">TNCB<\/jats:styled-content>to the ear. The<jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>2 antagonist<jats:styled-content style=\"fixed-case\">SB<\/jats:styled-content>225002 (9\u00a0mg\/kg, i.p.) was administered before each<jats:styled-content style=\"fixed-case\">TNCB<\/jats:styled-content>challenge. Gene expressions and protein levels of the<jats:styled-content style=\"fixed-case\">ELR<\/jats:styled-content><jats:sup>+<\/jats:sup>chemokines<jats:styled-content style=\"fixed-case\">CXCL<\/jats:styled-content>1, 2 and 5 was increased markedly in mouse ear after the final<jats:styled-content style=\"fixed-case\">TNCB<\/jats:styled-content>challenge. In addition, we indicated that gene expression of<jats:styled-content style=\"fixed-case\">CXCL<\/jats:styled-content>1 was enhanced in the epidermis and dermis upon<jats:styled-content style=\"fixed-case\">TNCB<\/jats:styled-content>challenge. Expression of the<jats:styled-content style=\"fixed-case\">CXCL<\/jats:styled-content>2 gene was enhanced in the epidermis, and that of the<jats:styled-content style=\"fixed-case\">CXCL<\/jats:styled-content>5 gene was enhanced in the dermis. The swelling induced by<jats:styled-content style=\"fixed-case\">TNCB<\/jats:styled-content>challenges was significantly attenuated by<jats:styled-content style=\"fixed-case\">SB<\/jats:styled-content>225002. Furthermore, the increases in myeloperoxidase activity, and expression of myeloperoxidase and neutrophil elastase induced by<jats:styled-content style=\"fixed-case\">TNCB<\/jats:styled-content>challenge in mouse ear were inhibited by<jats:styled-content style=\"fixed-case\">SB<\/jats:styled-content>225002. These data suggest that ear swelling resulting from<jats:styled-content style=\"fixed-case\">TNCB<\/jats:styled-content>challenges might be concerned by upregulated<jats:styled-content style=\"fixed-case\">ELR<\/jats:styled-content><jats:sup>+<\/jats:sup>chemokine\u2010induced neutrophil recruitment.<\/jats:p>","DOI":"10.1111\/1440-1681.12839","type":"journal-article","created":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T11:23:06Z","timestamp":1501586586000},"page":"27-33","source":"Crossref","is-referenced-by-count":7,"title":["<scp>ELR<\/scp><sup>+<\/sup>chemokine\u2010mediated neutrophil recruitment is involved in 2,4,6\u2010trinitrochlorobenzene\u2010induced contact hypersensitivity"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4501-2836","authenticated-orcid":false,"given":"Hiroyasu","family":"Sakai","sequence":"first","affiliation":[{"name":"Department of Analytical Pathophysiology School of Pharmacy Hoshi University Shinagawa\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saori","family":"Yabe","sequence":"additional","affiliation":[{"name":"Department of Analytical Pathophysiology School of Pharmacy Hoshi University Shinagawa\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Analytical Pathophysiology School of Pharmacy Hoshi University Shinagawa\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Kai","sequence":"additional","affiliation":[{"name":"Department of Analytical Pathophysiology School of Pharmacy Hoshi University Shinagawa\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumiaki","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Analytical Pathophysiology School of Pharmacy Hoshi University Shinagawa\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuro","family":"Yumoto","sequence":"additional","affiliation":[{"name":"Department of Analytical Pathophysiology School of Pharmacy Hoshi University Shinagawa\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuka","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Pharmacy Hoshi University Shinagawa\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minoru","family":"Narita","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Pharmacy Hoshi University Shinagawa\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenjiro","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Experimental Therapeutics Division of Pathological Sciences Kyoto Pharmaceutical University Yamashina Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8280-7558","authenticated-orcid":false,"given":"Shinichi","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Experimental Therapeutics Division of Pathological Sciences Kyoto Pharmaceutical University Yamashina Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiko","family":"Chiba","sequence":"additional","affiliation":[{"name":"Department of Physiology and Molecular Sciences School of Pharmacy Hoshi University Shinagawa\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-5769(01)00173-4"},{"key":"e_1_2_7_3_1","first-page":"564","article-title":"Skin diseases with high public health impact. 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