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The strong anti\u2010oxidative capacity of resveratrol has been demonstrated previously, but its role in the context of male reproduction remains inconclusive. To explore the biological role of resveratrol in protecting male reproductive function and the potential underlying mechanism, nicotine\u2010induced Leydig cells were used as a cell model of oxidative damage. The data showed that resveratrol treatment increased cell viability, <jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content> activity and anti\u2010apoptotic activity in nicotine\u2010stressed Leydig cells. This effect was accompanied by the upregulation of autophagy, which was illustrated by <jats:styled-content style=\"fixed-case\">MDC<\/jats:styled-content>\u2010LysoTracker red staining. Moreover, pretreating with 3\u2010methyladenine (3\u2010<jats:styled-content style=\"fixed-case\">MA<\/jats:styled-content>), an autophagy inhibitor, attenuated resveratrol\u2010induced Leydig cells autophagy and promoted apoptosis. Apart from this, resveratrol enhanced <jats:styled-content style=\"fixed-case\">AMPK<\/jats:styled-content> phosphorylation but reduced <jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content> phosphorylation. Subsequently, upon inhibiting <jats:styled-content style=\"fixed-case\">AMPK<\/jats:styled-content> phosphorylation by <jats:styled-content style=\"fixed-case\">AMPK<\/jats:styled-content> inhibitors, Leydig cell autophagy induced by resveratrol was obviously abolished. In conclusion, resveratrol may exert its cytoprotective role against oxidative injury by the activation of autophagy via <jats:styled-content style=\"fixed-case\">AMPK<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content> pathway.<\/jats:p>","DOI":"10.1111\/1440-1681.12895","type":"journal-article","created":{"date-parts":[[2017,11,22]],"date-time":"2017-11-22T06:36:40Z","timestamp":1511332600000},"page":"573-580","source":"Crossref","is-referenced-by-count":26,"title":["Resveratrol protects Leydig cells from nicotine\u2010induced oxidative damage through enhanced autophagy"],"prefix":"10.1111","volume":"45","author":[{"given":"Shengxian","family":"Liu","sequence":"first","affiliation":[{"name":"Centre of Reproductive Medicine Luoyang Central Hospital Affiliated to Zhengzhou University  Luoyang Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuqin","family":"Sun","sequence":"additional","affiliation":[{"name":"The 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