{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T09:11:03Z","timestamp":1777540263997,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,11,14]],"date-time":"2018-11-14T00:00:00Z","timestamp":1542153600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Shaanxi Province Key Scientific And Technological Project","award":["2012K16-01-02"],"award-info":[{"award-number":["2012K16-01-02"]}]},{"name":"Science and Technology Planning Project of Xi'an","award":["SF1202(6)"],"award-info":[{"award-number":["SF1202(6)"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The current study aimed to investigate the effects of sildenafil and N\u2010acetylcysteine (<jats:styled-content style=\"fixed-case\">NAC<\/jats:styled-content>) on the haemodynamics in a rabbit model of acute pulmonary thromboembolism (<jats:styled-content style=\"fixed-case\">APT<\/jats:styled-content>). We developed an <jats:styled-content style=\"fixed-case\">APT<\/jats:styled-content> model using healthy male China big\u2010ear rabbits (2.7\u00a0\u00b1\u00a00.4\u00a0kg). The rabbits were divided into five groups subjected to various interventions. We recorded the haemodynamic parameters and assessed the oxidative stress and lipid peroxidation response in the groups. Additionally, we detected apoptosis\u2010associated molecules, FoxO1, Bad and Bcl\u20102, in the lung tissue. Gelatine zymography was used to detect matrix metalloproteinase (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>) activity in bronchoalveolar lavage (<jats:styled-content style=\"fixed-case\">BLA<\/jats:styled-content>). Pulmonary artery endothelial cells were isolated, and their apoptosis rates and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content> activity were assayed. N\u2010acetylcysteine potentiated the haemodynamic\u2010improving effect of sildenafil and significantly inhibited the oxidative stress response. N\u2010acetylcysteine combined with sildenafil decreased <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20102 and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 activity and <jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content> consumption and inhibited apoptosis of pulmonary arterial endothelial cells. Moreover, <jats:styled-content style=\"fixed-case\">NAC<\/jats:styled-content> combined with sildenafil inhibited the expression of <jats:styled-content style=\"fixed-case\">MCP<\/jats:styled-content>\u20101 and p\u2010p38 <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content>. Thus, <jats:styled-content style=\"fixed-case\">NAC<\/jats:styled-content> potentiates the haemodynamic\u2010improving effect of sildenafil in a rabbit model of acute pulmonary thromboembolism via the <jats:styled-content style=\"fixed-case\">MCP<\/jats:styled-content>\u20101 and p38 <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> signalling pathway. This study may provide a promising treatment method for <jats:styled-content style=\"fixed-case\">APT<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/1440-1681.13039","type":"journal-article","created":{"date-parts":[[2018,10,6]],"date-time":"2018-10-06T10:05:10Z","timestamp":1538820310000},"page":"163-172","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["N\u2010Acetylcysteine potentiates the haemodynamic\u2010improving effect of sildenafil in a rabbit model of acute pulmonary thromboembolism via the p38 <scp>MAPK<\/scp> pathway"],"prefix":"10.1111","volume":"46","author":[{"given":"Ruipeng","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Peripheral Angiopathy The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"},{"name":"Department of Vascular Surgery Shaanxi Provincial People's Hospital Xi'an Shaanxi 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