{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T14:20:26Z","timestamp":1763389226222,"version":"3.40.5"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,10,8]],"date-time":"2019-10-08T00:00:00Z","timestamp":1570492800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31371152"],"award-info":[{"award-number":["31371152"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>It is known that 7,8\u2010dihydroxyflavone (7,8\u2010<jats:styled-content style=\"fixed-case\">DHF<\/jats:styled-content>), a synthetic agonist specific for TrkB, promotes intestinal cholinergic contraction. However, after intestinal ischaemia\u2013reperfusion (<jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>) injury, how 7,8\u2010<jats:styled-content style=\"fixed-case\">DHF<\/jats:styled-content> affects intestinal contractile dynamics is unknown. In this study, an <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content> injury model was prepared with rats subjected to 45\u00a0minutes clamping of the superior mesenteric artery. The <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content> injury decreased postoperative food intake and body weight, delayed defecation time, lowered intestinal propulsive rate and decreased cholinergic contraction of jejunal muscle strips, indicating the occurrence of injured jejunal contraction after <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>. Feeding rats with 7,8\u2010<jats:styled-content style=\"fixed-case\">DHF<\/jats:styled-content> improved these intestinal activities injured by <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>, which exhibited the in vivo effect of 7,8\u2010<jats:styled-content style=\"fixed-case\">DHF<\/jats:styled-content>. To explore its molecular mechanism, the expression and phosphorylation of TrkB, <jats:styled-content style=\"fixed-case\">PLC<\/jats:styled-content> \u03b31, Akt, and <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>1\/2 in the jejunal strips were examined with western blots. The <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content> injury significantly decreased the expression and phosphorylation levels of all factors studied here. However, 7,8\u2010<jats:styled-content style=\"fixed-case\">DHF<\/jats:styled-content> feeding specifically enhanced the phosphorylation of TrkB, <jats:styled-content style=\"fixed-case\">PLC<\/jats:styled-content> \u03b31 and Akt factors in both sham\u2010 and <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>\u2010operated rats, indicating that 7,8\u2010<jats:styled-content style=\"fixed-case\">DHF<\/jats:styled-content> may have activated TrkB which then activated its downstream <jats:styled-content style=\"fixed-case\">PLC<\/jats:styled-content> \u03b31 and Akt. Finally, we found that 7,8\u2010<jats:styled-content style=\"fixed-case\">DHF<\/jats:styled-content> augmented cholinergic receptor M3 expression somehow. These results imply a possibility that 7,8\u2010<jats:styled-content style=\"fixed-case\">DHF<\/jats:styled-content> might be capable of alleviating the jejunal contractile damage caused by <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content> through activation of TrkB and augmentation of M3 expression.<\/jats:p>","DOI":"10.1111\/1440-1681.13136","type":"journal-article","created":{"date-parts":[[2019,7,17]],"date-time":"2019-07-17T07:48:59Z","timestamp":1563349739000},"page":"67-75","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of 7,8\u2010dihydroxyflavone on rat jejunal dynamics subjected to ischaemia\u2013reperfusion injury"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8405-3895","authenticated-orcid":false,"given":"Li","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Clinical Nutrition Affiliated Hospital Qingdao University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiqiang","family":"Qu","sequence":"additional","affiliation":[{"name":"Center for Medical Research Affiliated Hospital Qingdao University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Luan","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Medical College of Qingdao University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuli","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Clinical Nutrition Affiliated Hospital Qingdao University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongfang","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Clinical Nutrition Affiliated Hospital Qingdao University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Clinical Nutrition Affiliated Hospital Qingdao University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Clinical Nutrition Affiliated Hospital Qingdao University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baoguo","family":"He","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Affiliated Hospital Qingdao University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,10,8]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_6_2_1","DOI":"10.3164\/jcbn.17-137"},{"key":"e_1_2_6_3_1","first-page":"18074","article-title":"Histopathology of human small\u00a0intestinal\u00a0and 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