{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T17:06:28Z","timestamp":1778864788712,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T00:00:00Z","timestamp":1567123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81622007"],"award-info":[{"award-number":["81622007"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870362"],"award-info":[{"award-number":["81870362"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Isoliquiritigenin (<jats:styled-content style=\"fixed-case\">ISL<\/jats:styled-content>) is a flavonoid substance with a chalcone structure, which exerts anti\u2010tumour, anti\u2010oxidation and anti\u2010inflammatory activity. The large\u2010conductance calcium\u2010activated potassium channel (<jats:styled-content style=\"fixed-case\">BK<jats:sub>C<\/jats:sub><\/jats:styled-content><jats:sub>a<\/jats:sub>) is an important potassium channel with negative feedback regulation on the vascular smooth muscle cells (<jats:styled-content style=\"fixed-case\">VSMC<\/jats:styled-content>s) membrane. The activation of <jats:styled-content style=\"fixed-case\">BK<jats:sub>C<\/jats:sub><\/jats:styled-content><jats:sub>a<\/jats:sub> channel causes the hyperpolarization of <jats:styled-content style=\"fixed-case\">VSMC<\/jats:styled-content>s. It plays an important role in relaxation of blood vessels. Previous studies have shown that <jats:styled-content style=\"fixed-case\">ISL<\/jats:styled-content> causes the relaxation of the aorta and the basilar artery of the rat. However, there have not been studies on regulation of <jats:styled-content style=\"fixed-case\">ISL<\/jats:styled-content> in mesenteric arteries. To examine whether <jats:styled-content style=\"fixed-case\">ISL<\/jats:styled-content> causes the relaxation of the mesenteric artery of mice, we recorded vasodilation of mouse mesenteric arterial rings with a myograph. After contraction of arterial rings with phenylephrine, we added <jats:styled-content style=\"fixed-case\">ISL<\/jats:styled-content> to the arterial rings and measured its relaxation effect. To further examine which channel was involved in this relaxation effect, we tested the effects of <jats:styled-content style=\"fixed-case\">ISL<\/jats:styled-content> on endothelium\u2010dependent and endothelium\u2010independent vasodilation. Then we used <jats:styled-content style=\"fixed-case\">BK<jats:sub>C<\/jats:sub><\/jats:styled-content><jats:sub>a<\/jats:sub> channel blockers tetraethylammonium and iberiotoxin, to detect whether the <jats:styled-content style=\"fixed-case\">BK<jats:sub>C<\/jats:sub><\/jats:styled-content><jats:sub>a<\/jats:sub> channel is involved in <jats:styled-content style=\"fixed-case\">ISL<\/jats:styled-content>\u2010induced vasodilation. Mesenteric arterial smooth muscle cells were isolated by enzyme digestion. Bis\u2010(1, 3\u2010dibutylbarbituric acid) trimethine oxonol staining was used to measure membrane potential of mesenteric arterial smooth muscle cells. We identified a vasodilation effect caused by <jats:styled-content style=\"fixed-case\">ISL<\/jats:styled-content> on mouse mesenteric arterial rings pre\u2010contracted by phenylephrine in a concentration\u2010dependent manner, with an <jats:styled-content style=\"fixed-case\">EC<\/jats:styled-content><jats:sub>50<\/jats:sub> of 13.71\u00a0\u00b1\u00a01.1\u00a0\u03bcmol\/L. The vasodilation effect of <jats:styled-content style=\"fixed-case\">ISL<\/jats:styled-content> is endothelium\u2010independent. K<jats:sup>+<\/jats:sup> channel inhibitors tetraethylammonium and iberiotoxin reduced the vasodilation induced by <jats:styled-content style=\"fixed-case\">ISL<\/jats:styled-content> which suggested the involvement of <jats:styled-content style=\"fixed-case\">BK<jats:sub>C<\/jats:sub><\/jats:styled-content><jats:sub>a<\/jats:sub> channel.<\/jats:p>","DOI":"10.1111\/1440-1681.13156","type":"journal-article","created":{"date-parts":[[2019,8,8]],"date-time":"2019-08-08T18:18:37Z","timestamp":1565288317000},"page":"1044-1052","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Isoliquiritigenin\u2010induced vasodilation by activating large\u2010conductance Ca<sup>2+<\/sup>\u2010activated K<sup>+<\/sup> channels in mouse mesenteric arteries"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8426-6014","authenticated-orcid":false,"given":"Yang","family":"Ye","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences Jiangnan University Wuxi Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengru","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Jiangnan University Wuxi Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Feng","sequence":"additional","affiliation":[{"name":"Wuxi School of Medicine Jiangnan University Wuxi Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bainian","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Jiangnan University Wuxi Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8525-068X","authenticated-orcid":false,"given":"Xin","family":"Ma","sequence":"additional","affiliation":[{"name":"Wuxi School of Medicine Jiangnan University Wuxi Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,8,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2009.00052.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.51.030189.002125"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.268.4.C799"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.02.045"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2012.04.002"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.57.4.9"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2004.08.045"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1996.00440060059007"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)92876-U"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408399709527802"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/26.1.1"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-8223(96)00273-8"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb14943.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(92)90076-G"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-962477"},{"key":"e_1_2_8_17_1","volume-title":"Comprehensive Physiology","author":"Davis MJ","year":"2008"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0896(97)10034-7"},{"issue":"14","key":"e_1_2_8_19_1","first-page":"2916","article-title":"Flavonoids, dietary\u2010derived inhibitors of cell proliferation and in vitro angiogenesis","volume":"57","author":"Fotsis T","year":"1997","journal-title":"Can Res"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(79)90134-1"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.52.2.137"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.281.6.C1769"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.67.2.525"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.84.9.1032"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00040.2005"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2012.04.002"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.00181.x"}],"container-title":["Clinical and Experimental Pharmacology and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1440-1681.13156","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1440-1681.13156","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1440-1681.13156","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T12:22:56Z","timestamp":1694262176000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1440-1681.13156"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,30]]},"references-count":26,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1111\/1440-1681.13156"],"URL":"https:\/\/doi.org\/10.1111\/1440-1681.13156","archive":["Portico"],"relation":{},"ISSN":["0305-1870","1440-1681"],"issn-type":[{"value":"0305-1870","type":"print"},{"value":"1440-1681","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,30]]},"assertion":[{"value":"2019-04-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}