{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T20:59:08Z","timestamp":1771880348444,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,1,28]],"date-time":"2020-01-28T00:00:00Z","timestamp":1580169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Asparaginase is fundamental to the treatment of haematological malignancies. However, little has been studied on the effects that asparaginase could exert on solid tumours. Thus, this study aimed to evaluate the effects of asparaginase on an oral carcinoma cell line. The cytotoxicity of asparaginase in SCC\u20109 (tongue squamous cell carcinoma) and HaCaT (human keratinocyte) cell lines was evaluated with MTT cell viability assay. The cells were treated with asparaginase at 0.04, 0.16, 0.63, 1.0, 1.5, 2.5, and 5.0\u00a0IU\/mL. Dose\u2010response curves and IC<jats:sub>50<\/jats:sub> values were obtained and the Tumour Selectivity Index (TSI) was calculated. The effect of asparaginase on procaspase\u20103 and nuclear factor \u03baB (NF\u03baB) expression was evaluated with western blot because it was reported that the overexpression of NF\u03baB has been shown to contribute to tumour cell survival, proliferation, and migration. Caspase 3\/7 staining was performed to identify cell death using flow cytometry. Effective asparaginase concentrations were lower for SCC\u20109 cells when compared to HaCaT cells. The cytotoxicity results at 48 and 72\u00a0hours were significantly different for SCC\u20109 cells. The TSI indicated that asparaginase was selective for the tumour cells. A decrease in procaspase\u20103 and NF\u03baB protein levels was observed in SCC\u20109 cells. Furthermore, asparaginase resulted in significant apoptosis after 48 and 72\u00a0hours. Based on these results, asparaginase was cytotoxic in a dose\u2010 and time\u2010dependent manner, induces apoptosis, and reduces NF\u03baB expression in oral cancer cells. These results encourage further studies on the effectiveness of this enzyme as a treatment for solid tumours, especially head and neck cancer.<\/jats:p>","DOI":"10.1111\/1440-1681.13256","type":"journal-article","created":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T14:18:30Z","timestamp":1578925110000},"page":"857-866","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Asparaginase induces selective dose\u2010 and time\u2010dependent cytotoxicity, apoptosis, and reduction of NF\u03baB expression in oral cancer cells"],"prefix":"10.1111","volume":"47","author":[{"given":"Gabriel \u00c1lvares","family":"Borges","sequence":"first","affiliation":[{"name":"Laboratory of Oral Histopathology Faculty of Health Sciences University of Brasilia Brasilia Brazil"},{"name":"Epithelial Biology Laboratory Department of Periodontics and Oral Medicine Division of Oral Pathology Oral Radiology and Oral Medicine University of Michigan School of Dentistry Ann Arbor MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia Taveira","family":"Elias","sequence":"additional","affiliation":[{"name":"Laboratory of Oral Histopathology Faculty of Health Sciences University of Brasilia Brasilia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tassiana Souza De","family":"Araujo","sequence":"additional","affiliation":[{"name":"Laboratory of Oral Histopathology Faculty of Health Sciences University of Brasilia Brasilia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula Monteiro","family":"Souza","sequence":"additional","affiliation":[{"name":"Natural Products Laboratory Faculty of Health Sciences University of Brasilia Brasilia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Henrique Viesi","family":"Nascimento\u2010Filho","sequence":"additional","affiliation":[{"name":"Epithelial Biology Laboratory Department of Periodontics and Oral Medicine Division of Oral Pathology Oral Radiology and Oral Medicine University of Michigan School of Dentistry Ann Arbor MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rogerio M.","family":"Castilho","sequence":"additional","affiliation":[{"name":"Epithelial Biology Laboratory Department of Periodontics and Oral Medicine Division of Oral Pathology Oral Radiology and Oral Medicine University of Michigan School of Dentistry Ann Arbor MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristiane H.","family":"Squarize","sequence":"additional","affiliation":[{"name":"Epithelial Biology Laboratory Department of Periodontics and Oral Medicine Division of Oral Pathology Oral Radiology and Oral Medicine University of Michigan School of Dentistry Ann Arbor MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P\u00e9rola de Oliveira","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Natural Products Laboratory Faculty of Health Sciences University of Brasilia Brasilia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7622-1550","authenticated-orcid":false,"given":"Eliete Neves Silva","family":"Guerra","sequence":"additional","affiliation":[{"name":"Laboratory of Oral Histopathology Faculty of Health Sciences University of Brasilia Brasilia Brazil"},{"name":"Epithelial Biology Laboratory Department of Periodontics and Oral Medicine Division of Oral Pathology Oral Radiology and Oral Medicine University of Michigan School of Dentistry Ann Arbor MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,1,28]]},"reference":[{"issue":"12","key":"e_1_2_7_2_1","first-page":"2912","article-title":"Dietary acrylamide and cancer risk: an updated meta\u2010analysis","volume":"136","author":"Pelucchi C","year":"2015","journal-title":"Drugs"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2016.04.105"},{"key":"e_1_2_7_4_1","unstructured":"WHO. 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