{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:39:01Z","timestamp":1772159941610,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,2,9]],"date-time":"2020-02-09T00:00:00Z","timestamp":1581206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Acne vulgaris is a chronic inflammatory disease of the skin resulting from androgen\u2010induced increased sebum production and altered keratinization. Nicotinamide (NAM), an amide form of vitamin B3 with a well\u2010established safety profile, has shown good therapeutic potential in treating acne and its complications. NAM has anti\u2010inflammatory effects and reduces sebum but its function in androgen biosynthesis remains unknown. In this study, we used a widely used cell model, starved human adrenal NCI\u2010H295R cells, to examine the effects of NAM in androgen production and its mediated network changes. By treating NCI\u2010H295R cells with 1\u201025\u00a0mmol\/L of NAM, we found that cell viability was only slightly inhibited at the highest dose (25\u00a0mmol\/L). NAM reduced testosterone production in a dose\u2010dependent manner. Transcriptomic analysis demonstrated that key enzymes of androgen biosynthesis were significantly decreased under NAM treatment. In addition, gene set enrichment analysis (GSEA) showed that gene sets of cell cycle, steroid biosynthesis, TGF\u03b2 signalling, and targets of IGF1 or IGF2 were enriched in NAM\u2010treated cells. Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway and Gene ontology (GO) analysis of the differentially expressed genes also suggested that steroidogenesis and SMAD signalling were affected by NAM. Overall, these crucial genes and pathways might form a complex network in NAM\u2010treated NCI\u2010H295R cells and result in androgen reduction. These findings help explain the potential molecular actions of NAM in acne vulgaris, and position NAM as a candidate for the treatment of other hyperandrogenic disorders.<\/jats:p>","DOI":"10.1111\/1440-1681.13258","type":"journal-article","created":{"date-parts":[[2020,1,18]],"date-time":"2020-01-18T13:29:41Z","timestamp":1579354181000},"page":"821-830","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["An integrated RNA\u2010Seq and network study reveals the effect of nicotinamide on adrenal androgen synthesis"],"prefix":"10.1111","volume":"47","author":[{"given":"Xueying","family":"Gao","sequence":"first","affiliation":[{"name":"Center for Reproductive Medicine Shandong University Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiheng","family":"Yu","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine Shandong University Jinan 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