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The anti\u2010malarial drug artesunate (ART) also exerts anticancer effects in various cancers; however, the combined effect of ART and CBP on non\u2010small cell lung cancer (NSCLC) remains unclear. In the present study, the NSCLC cell line A549 was pretreated with various concentrations of CBP, ART and gemcitabine (GEM). 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide (MTT) assays were conducted to detect cell viability. Cell apoptosis was evaluated by both flow cytometry and TUNEL apoptotic assay. The expression profiles of cell cycle\u2010related proteins and apoptotic proteins were determined by western blot. Cell clone numbers were visualized using crystal violet staining. Here, we found that both CBP and ART suppressed cell viability, and promoted cell apoptosis, and the combined application of ART and CBP at a lower concentration exhibited synergistic effects. Specifically, the combination of ART and CBP at a lower concentration suppressed cell clone numbers, promoted cell cycle arrest at the G<jats:sub>2<\/jats:sub>\/M phase, and induced the expression of the cell cycle and apoptosis\u2010related proteins BAX, p21, p53, and Caspase\u20103, while decreasing Bcl\u20102 and Cyclin B1 expression. Based on these results, we concluded that combined application of ART and CBP exerts synergistic anti\u2010tumour effects on NSCLC by enhancing cell apoptosis in a mitochondria\u2010dependent\u00a0manner.<\/jats:p>","DOI":"10.1111\/1440-1681.13287","type":"journal-article","created":{"date-parts":[[2020,2,19]],"date-time":"2020-02-19T08:11:07Z","timestamp":1582099867000},"page":"1083-1091","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["The combination of artesunate and carboplatin exerts a synergistic anti\u2010tumour effect on non\u2010small cell lung cancer"],"prefix":"10.1111","volume":"47","author":[{"given":"Zhu","family":"Wang","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Diagnosis of Cancer West China Hospital of Sichuan University Chengdu 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