{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T16:05:45Z","timestamp":1781712345109,"version":"3.54.5"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T00:00:00Z","timestamp":1605571200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004000","name":"Guangzhou Science and Technology Program key projects","doi-asserted-by":"publisher","award":["201804010054"],"award-info":[{"award-number":["201804010054"]}],"id":[{"id":"10.13039\/501100004000","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Lesions of the peripheral nerves can lead to lifelong neuropathic pain (NP). Autophagic deficiency in the Schwann cells (SCs) is an early event in the origin of NP chronification. Uncoordinated gene 5H2 (UNC5H2), one of the repulsive netrin receptors, mediated the effect of netrin\u20101 on autophagic activation and cell survival in endothelial cells. However, its role on autophagy regulation in peripheral nerves during NP process remains unidentified. Chronic constriction injury (CCI) of the left sciatic nerve was induced in Sprague\u2010Dawley rats, and UNC5H2 small interfering RNA was transfected to the ipsilateral sciatic nerve immediately after injury. Mechanical allodynia was assessed. Sciatic UNC5H2 and netrin\u20101 protein levels were investigated. Autophagy in the ipsilateral sciatic nerves was evaluated by detecting punctate light chain 3(LC3) and autophagosomes, as well as the levels of LC3 II, p62 and phosphorylated UNC51\u2010like kinase (ULK1). After CCI, UNC5H2 of the sciatic nerves was upregulated, exclusively expressed in SCs. Small interfering RNA transfection resulted in significant decrease of UNC5H2 and netrin\u20101 protein, leading to exaggeration of mechanical allodynia through 14\u00a0days after CCI. Autophagy was activated but autophagic influx was interfered within a week after CCI, shown by the elevated levels of both LC3II and p62, which was further deteriorated with UNC5H2 knockdown. In addition, the injury\u2010induced augmentation of phosphorylated ULK1 was significantly diminished by UNC5H2 knockdown. Altogether, the results suggest that local UNC5H2 of the peripheral nerve plays a significant role in the process of injury\u2010induced mechanical allodynia, probably associated to its contribution to autophagic regulation.<\/jats:p>","DOI":"10.1111\/1440-1681.13430","type":"journal-article","created":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T22:13:42Z","timestamp":1604009622000},"page":"361-369","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Local uncoordinated gene 5H2 contributes to nerve injury\u2010induced mechanical allodynia associated to its role in autophagy"],"prefix":"10.1111","volume":"48","author":[{"given":"Jing\u2010Yan","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital Sun Yat\u2010sen University Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Diagnosis and Treatment of Major Neurological Diseases National Key Clinical Department and Key Discipline of Neurology Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital Sun Yat\u2010sen University Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Diagnosis and Treatment of Major Neurological Diseases National Key Clinical Department and Key Discipline of Neurology Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei\u2010Yao","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital Sun Yat\u2010sen University Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Diagnosis and Treatment of Major Neurological Diseases National Key Clinical Department and Key Discipline of Neurology Guangzhou 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