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Empagliflozin (EMPA), is a novel hypoglycaemic drug which possesses multiple pharmacologically relevant protective effects, including anti\u2010apoptotic, anti\u2010inflammatory and antioxidant effects. However, whether EMPA treatment has a protective effect on ethanol\u2010induced myocardial injury has not been assessed, to the best of our knowledge. Therefore, the aim of this study was to determine the effect of EMPA treatment on ethanol\u2010induced myocardial injury and the underlying mechanism. An ethanol\u2010induced myocardial injury model was established by culturing H9c2 cells treated with 200\u00a0mmol\/L ethanol for 24\u00a0hours, and additional groups of ethanol treated cells were also treated with EMPA with or without SIRT1 inhibitors prior to ethanol treatment. Cell viability and apoptosis were assessed using a CCK\u20108 assay and flow cytometry, respectively. The expression of apoptosis\u2010related proteins was assessed using western blotting. The results showed that EMPA pretreatment resulted in increased cell viability and a decrease in LDH activity. Moreover, EMPA pretreatment significantly reduced apoptosis of cardiomyocytes, and reduced the expression of cleaved caspase 3. Furthermore, EMPA increased the expression of SIRT1, increased the phosphorylation levels of Akt, and reduced the expression of PTEN. EMPA also reduced ethanol\u2010induced mitochondrial apoptosis, increasing the Bcl\u20102\/Bax ratio and the mitochondrial membrane potential. However, the cardioprotective effects of EMPA were abrogated when cells were pretreated with a SIRT1 inhibitor. In conclusion, EMPA can alleviate ethanol\u2010induced myocardial injury by inhibiting mitochondrial apoptosis via the SIRT1\/PTEN\/Akt pathway. Therefore, EMPA may be a novel target for treatment of ethanol\u2010induced myocardial injury.<\/jats:p>","DOI":"10.1111\/1440-1681.13470","type":"journal-article","created":{"date-parts":[[2021,2,3]],"date-time":"2021-02-03T23:01:54Z","timestamp":1612393314000},"page":"837-845","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Empagliflozin alleviates ethanol\u2010induced cardiomyocyte injury through inhibition of mitochondrial apoptosis via a SIRT1\/PTEN\/Akt pathway"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8685-1329","authenticated-orcid":false,"given":"Ge","family":"Tian","sequence":"first","affiliation":[{"name":"Department of Cardiology The First Affiliated Hospital of China Medical University Shenyang China"},{"name":"Department of Cardiology The First Affiliated Hospital of Jinzhou Medical University Jinzhou 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