{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T02:26:10Z","timestamp":1768530370045,"version":"3.49.0"},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,2,25]],"date-time":"2021-02-25T00:00:00Z","timestamp":1614211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Properties of mutant human ether\u2010\u00e0\u2010go\u2010go\u2010related gene (hERG) channels can be modified by some antibiotics. However, the pharmacological effects of posaconazole on cardiomyocyte hERG channels remain unclear. Whole\u2010cell patch clamping, western blotting and laser confocal scanning microscopy were used to evaluate the effects of posaconazole on wild\u2010type (WT)\u2010, A561V\u2010 and L539\u00a0fs\/47\u2010hERG channels expressed in human embryonic kidney (HEK) 293 cells. In electrophysiological experiments, HEK 293 cells were transiently co\u2010transfected with equal amounts of WT\u2010hERG, WT+A561\u00a0V\u2010hERG and WT+L539\u00a0fs\/47\u2010hERG plasmids to mimic a heterozygous genotype. Posaconazole (30\u00a0\u03bcM) increased tail currents in cells expressing WT\u2010hERG, WT+A561\u00a0V\u2010hERG and WT+L539\u00a0fs\/47\u2010hERG by 82.65%, 147.72% and 134.73%, respectively, compared to controls. Posaconazole increased hERG protein expression in cells expressing WT\u2010hERG, WT+A561\u00a0V\u2010hERG and WT+L539\u00a0fs\/47\u2010hERG compared to controls condition as well as their trafficking to the cell membrane. To our knowledge, this is the first study to show that antifungal agent posaconazole rescues the mutant A561\u00a0V\u2010hERG and L539\u00a0fs\/47\u2010hERG channels by altering the gating kinetics, enhancing the expression and trafficking of hERG channels. The results demonstrate that posaconazole could be a promising candidate for the prevention and treatment of long QT syndrome and other arrhythmia\u2010related diseases.<\/jats:p>","DOI":"10.1111\/1440-1681.13475","type":"journal-article","created":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T00:30:52Z","timestamp":1614299452000},"page":"855-868","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Pharmacological corrections of the mutant hERG channels by posaconazole"],"prefix":"10.1111","volume":"48","author":[{"given":"Chaodi","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Cardiology The First Affiliated Hospital of Xi'an Jiaotong University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinglong","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery The First Affiliated Hospital of Xi'an Jiaotong University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery The First Affiliated Hospital of Xi'an Jiaotong University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongjian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery The First Affiliated Hospital of Xi'an Jiaotong University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery The First Affiliated Hospital of Xi'an Jiaotong University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery The First Affiliated Hospital of Xi'an Jiaotong University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery The First Affiliated Hospital of Xi'an Jiaotong University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21445"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joa.2015.11.009"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2016.4859"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1440-1681.12028"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/17425255.2017.1391213"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0035-1562902"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/1440-1681.12593"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000019513.50928.74"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2014.06.044"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.44.31123"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hrthm.2012.09.124"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1540-8167.2003.02363.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2003.08.002"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-7-93"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M305787200"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.210244497"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6535"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000079028.31393.15"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.279.4.H1748"}],"container-title":["Clinical and Experimental Pharmacology and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1440-1681.13475","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1440-1681.13475","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1440-1681.13475","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T01:02:19Z","timestamp":1693616539000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1440-1681.13475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,25]]},"references-count":19,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["10.1111\/1440-1681.13475"],"URL":"https:\/\/doi.org\/10.1111\/1440-1681.13475","archive":["Portico"],"relation":{},"ISSN":["0305-1870","1440-1681"],"issn-type":[{"value":"0305-1870","type":"print"},{"value":"1440-1681","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,25]]},"assertion":[{"value":"2020-10-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}