{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:13:39Z","timestamp":1762272819147},"reference-count":33,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,8,22]],"date-time":"2021-08-22T00:00:00Z","timestamp":1629590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study was presented to observe the therapeutic effects of azathioprine (AZA) pretreatment on myocardial ischaemia reperfusion (I\/R) damage in diabetic rats. All rats were randomly separated into control\u00a0+\u00a0sham operation; control +I\/R; diabetes mellitus (DM)\u00a0+I\/R and DM +I\/R\u00a0+\u00a0AZA groups. Diabetic rat models were established by intraperitoneally injecting 60\u00a0mg\/kg streptozotocin (STZ). Diabetic rats were given 3\u00a0mg\/kg AZA daily by gavage for 5\u00a0days. Then, myocardial I\/R rat models were constructed. Myocardial infarction size and myocardial damage were respectively detected by TTC and H&amp;E staining. Cardiac injury markers (CK\u2010MB and MPO) and oxidative stress factors (SOD and MDA) were measured via ELISA. The protein expression of apoptotic markers (Caspase8, Caspase3, BAX and Bcl2), inflammatory factors (TLR4 and TNF\u2010\u03b1) and AKT1\/GSK3\u03b2 in myocardial tissues was measured by western blot, immunohistochemistry or immunofluorescence. Data showed that AZA pretreatment could lessen myocardial infarction size and myocardial damage, and could down\u2010regulate serum CK\u2010MB, MPO, SOD and MDA levels in diabetic rats under I\/R. Furthermore, AZA pretreatment decreased Caspase8, Caspase3, BAX, TLR4 and TNF\u2010\u03b1 expression, and increased Bcl2 expression in myocardial tissues of diabetic rats following I\/R. Also, AZA pretreatment lowered AKT1, p\u2010AKT1, GSK3\u03b2 and p\u2010GSK3\u03b2 expression in diabetic heart after I\/R. This study found that AZA may reduce myocardial injury in diabetic rats following I\/R via reducing oxidative stress, cardiomyocyte apoptosis, and inflammatory response, which could be related to AKT1\/GSK3\u03b2 pathway inactivation.<\/jats:p>","DOI":"10.1111\/1440-1681.13569","type":"journal-article","created":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T20:03:10Z","timestamp":1628539390000},"page":"1621-1632","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Azathioprine pretreatment ameliorates myocardial ischaemia reperfusion injury in diabetic rats by reducing oxidative stress, apoptosis, and inflammation"],"prefix":"10.1111","volume":"48","author":[{"given":"Cuijie","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Basic Medicine Sichuan Vocational College of Health and Rehabilitation Zigong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Basic Medicine Sichuan Vocational College of Health and Rehabilitation Zigong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Basic Medicine Sichuan Vocational College of Health and Rehabilitation Zigong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junfei","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Basic Medicine Sichuan Vocational College of Health and Rehabilitation Zigong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Basic Medicine Sichuan Vocational College of Health and Rehabilitation Zigong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenxian","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Basic Medicine Sichuan Vocational College of Health and Rehabilitation Zigong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Basic Medicine Sichuan Vocational College of Health and Rehabilitation Zigong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,8,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.565160"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2019.5097"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9080679"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-03295-y"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/3732718"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/2719252"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12698"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-020-00526-y"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14993"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/6437467"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-020-0374-8"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2019.102372"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes10040277"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kez478"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2014.95"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2019.02.030"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20235853"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/4105382"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2019.03.004"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2019.01.001"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/6190504"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-018-1664-3"},{"issue":"3","key":"e_1_2_10_24_1","first-page":"1048","article-title":"Hydrogen\u2010rich saline alleviates inflammation and apoptosis in myocardial I\/R injury via PINK\u2010mediated autophagy","volume":"44","author":"Yao L","year":"2019","journal-title":"Int J Mol Med"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm.2019.10.108"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/17435390.2020.1731857"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2015.12.020"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.05.033"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2019.09.001"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-020-0776-4"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resuscitation.2012.04.001"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0b013e318186a84d"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(20)30070-3"},{"key":"e_1_2_10_34_1","first-page":"60783","article-title":"Establishment of acute pontine infarction in rats by electrical stimulation","volume":"162","author":"Luo M","year":"2020","journal-title":"J Vis Exp"}],"container-title":["Clinical and Experimental Pharmacology and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1440-1681.13569","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1440-1681.13569","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1440-1681.13569","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T10:31:02Z","timestamp":1693218662000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1440-1681.13569"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,22]]},"references-count":33,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1111\/1440-1681.13569"],"URL":"https:\/\/doi.org\/10.1111\/1440-1681.13569","archive":["Portico"],"relation":{},"ISSN":["0305-1870","1440-1681"],"issn-type":[{"value":"0305-1870","type":"print"},{"value":"1440-1681","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,22]]},"assertion":[{"value":"2021-03-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}