{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T18:43:10Z","timestamp":1779302590174,"version":"3.51.4"},"reference-count":77,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T00:00:00Z","timestamp":1632268800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771178"],"award-info":[{"award-number":["81771178"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2020JJ4553"],"award-info":[{"award-number":["2020JJ4553"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies reveal that hydrogen sulphide (H<jats:sub>2<\/jats:sub>S) exerts neuroprotection against neurotoxin\u2010induced Parkinson's disease (PD), but the underlying mechanism remains elusive. The present study was aimed to investigate whether H<jats:sub>2<\/jats:sub>S inhibits neuronal apoptosis of substantia nigra with the involvement of autophagy via promoting leptin signalling in 6\u2010hydroxydopamine (6\u2010OHDA)\u2010induced PD rats. In this study, neuronal apoptosis was analysed by TUNEL staining, the activity of caspase\u20103 was measured by Caspase\u20103\u00a0fluorometric assay kit, the expressions of Bax, Bcl\u20102, Beclin\u20101, LC3II, P62 and leptin were determined by Western blot analysis, and the numbers of autophagosomes and autolysosomes were assessed by transmission electron microscopy. Results showed that NaHS, a donor of exogenous H<jats:sub>2<\/jats:sub>S, mitigates 6\u2010OHDA\u2010induced the increases in the numbers of TUNEL\u2010positive cells, the activity of caspase\u20103 and the expression of Bax, and attenuates 6\u2010OHDA\u2010induced a decrease in the expression of Bcl\u20102 in substantia nigra of rats. In addition, 6\u2010OHDA enhanced the expressions of Beclin\u20101, LC3\u2010II and P62, increased the number of autophagosomes, and decreased the number of autolysosomes in the substantia nigra, which were also blocked by administration of NaHS. Furthermore, NaHS reversed 6\u2010OHDA\u2010induced the down\u2010regulation of leptin expression in the substantia nigra, and treatment with leptin\u2010OBR, a blocking antibody of leptin receptor, attenuated the inhibition of NaHS on neuronal apoptosis and the improvement of NaHS on the blocked autophagic flux in substantia nigra of 6\u2010OHDA\u2010treated rats. Taken together, these results demonstrated that H<jats:sub>2<\/jats:sub>S attenuates neuronal apoptosis of substantia nigra depending on restoring impaired autophagic flux through up\u2010regulating leptin signalling in PD.<\/jats:p>","DOI":"10.1111\/1440-1681.13587","type":"journal-article","created":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T08:24:31Z","timestamp":1631089471000},"page":"122-133","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Hydrogen sulphide attenuates neuronal apoptosis of substantia nigra by re\u2010establishing autophagic flux via promoting leptin signalling in a 6\u2010hydroxydopamine rat model of Parkinson's disease"],"prefix":"10.1111","volume":"49","author":[{"given":"Wu","family":"Jiang","sequence":"first","affiliation":[{"name":"The Affiliated Nanhua Hospital Department of Neurology Hengyang Medical School University of South China Hengyang Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zou","sequence":"additional","affiliation":[{"name":"The Affiliated Nanhua Hospital Department of Neurology Hengyang Medical School University of South China Hengyang Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Hu","sequence":"additional","affiliation":[{"name":"The Affiliated Nanhua Hospital Department of Neurology Hengyang Medical School University of South China Hengyang Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Tian","sequence":"additional","affiliation":[{"name":"Hengyang Key Laboratory of Neurodegeneration and Cognitive Impairment Institute of Neuroscience Hengyang Medical School University of South China Hengyang Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Xiao","sequence":"additional","affiliation":[{"name":"The Affiliated Nanhua Hospital Department of Neurology Hengyang Medical School University of South China Hengyang Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Li","sequence":"additional","affiliation":[{"name":"The Affiliated Nanhua Hospital Department of Neurology Hengyang Medical School University of South China Hengyang Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Affiliated Nanhua Hospital Department of Neurology Hengyang Medical School University of South China Hengyang Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong\u2010Jun","family":"Chen","sequence":"additional","affiliation":[{"name":"The Affiliated Nanhua Hospital Department of Neurology Hengyang Medical School University of South China Hengyang Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia\u2010Mei","family":"Jiang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital Institute of Neurology Hengyang Medical School University of South China Hengyang Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,9,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/18715273113126660155"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/01616412.2017.1390903"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.4074"},{"key":"e_1_2_8_5_1","first-page":"3789","article-title":"A new hope for a devastating disease: hydrogen sulfide in Parkinson's disease","volume":"55","author":"Cao X","year":"2018","journal-title":"Mol Neurobiol"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.108.047985"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00887.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09782"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.1972pi"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.5604\/01.3001.0010.4672"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/398483"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20170023"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867325666180226094351"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2018.04.013"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2018.11.001"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40263-018-0497-5"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.5834"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.38315"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.18"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c160041"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2015.06.001"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2014.04.012"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2009.12.033"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12640-009-9109-y"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1235-07.2007"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M705426200"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2019.00378"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2020.104692"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-014-4607-4"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.3347"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/2153745"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12017-018-8489-7"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-8-9"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19123999"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2014.11.004"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201600698R"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20170035"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncl.2014.09.010"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00568-3"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3671"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-020-03801-y"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina56060300"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.21571.1"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13731"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.15698\/cst2020.07.225"},{"key":"e_1_2_8_46_1","article-title":"Autophagy in neurodegenerative diseases: a hunter for aggregates","volume":"21","author":"Park H","year":"2020","journal-title":"Int J Mol Sci"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9051115"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110145"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a009357"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1920-10.2010"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5028"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.011163"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.060524"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2018.02.028"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/1440-1681.13201"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-018-2208-x"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2020.1741841"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.922032"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2019.00828"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/ane.12411"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.10.17.17067"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2014.06.010"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1080\/21623945.2019.1569447"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01507"},{"key":"e_1_2_8_65_1","first-page":"786","article-title":"Leptin inhibits apoptosis of nucleus pulposus cells via promoting autophagy","volume":"22","author":"Zhang W","year":"2018","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eplepsyres.2018.07.023"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.00541"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00963"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26043"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1111\/bpa.12370"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.008733"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(07)70327-7"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1002\/mds.26966"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes9050250"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.35878"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4043"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.04.001"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.573422"}],"container-title":["Clinical and Experimental Pharmacology and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1440-1681.13587","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1440-1681.13587","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1440-1681.13587","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T12:18:04Z","timestamp":1693225084000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1440-1681.13587"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,22]]},"references-count":77,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1111\/1440-1681.13587"],"URL":"https:\/\/doi.org\/10.1111\/1440-1681.13587","archive":["Portico"],"relation":{},"ISSN":["0305-1870","1440-1681"],"issn-type":[{"value":"0305-1870","type":"print"},{"value":"1440-1681","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,22]]},"assertion":[{"value":"2020-09-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}