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Thus, decreasing the activity of osteoclastic bone resorption is the main therapeutic method for treating osteoporosis. 2E\u2010Decene\u20104, 6\u2010diyn\u20101\u2010ol\u2010acetate (DDA) is a natural bioactive compound with anti\u2010inflammatory and anti\u2010cancer properties. However, its effects on osteoclastogenesis are unknown. Murine bone marrow\u2010derived macrophages (BMMs) or RAW264.7 cells were treated with DDA, followed by evaluation of cell viability, RANKL\u2010induced osteoclast differentiation, and pit formation assay. Effects of DDA on RANKL\u2010induced phosphorylation of MAPKs were assayed by western blot analysis. Expression of osteoclast\u2010specific genes was examined with reverse transcription\u2010PCR (RT\u2010PCR) and western blot analysis. In this study, DDA significantly inhibited RANKL\u2010induced osteoclast differentiation in RAW264.7 cells as well as in BMMs without cytotoxicity. DDA also strongly blocked the resorbing capacity of BMM on calcium phosphate\u2010coated plates. DDA inhibited RANKL\u2010induced phosphorylation of ERK, JNK and p38\u00a0MAPKs, as well as expression of c\u2010Fos and NFATc1, which are essential transcription factors for osteoclastogenesis. In addition, DDA decreased expression levels of osteoclastogenesis\u2010specific genes, including matrix metalloproteinase\u20109 (MMP\u20109), tartrate\u2010resistant acid phosphatase (TRAP), and receptor activator of NF\u2010\u03baB (RANK) in RANKL\u2010induced RAW264.7 cells. Collectively, these findings indicated that DDA attenuates RANKL\u2010induced osteoclast formation by suppressing the MAPKs\u2010c\u2010Fos\u2010NFATc1\u00a0signalling pathway and osteoclast\u2010specific genes. These results indicate that DDA may be a potential candidate for bone diseases associated with abnormal osteoclast formation and function.<\/jats:p>","DOI":"10.1111\/1440-1681.13609","type":"journal-article","created":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T05:46:09Z","timestamp":1635918369000},"page":"341-349","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["2E\u2010Decene\u20104,6\u2010diyn\u20101\u2010ol\u2010acetate inhibits osteoclastogenesis through mitogen\u2010activated protein kinase\u2010c\u2010Fos\u2010NFATc1 signalling pathways"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8263-5258","authenticated-orcid":false,"given":"Young Ran","family":"Park","sequence":"first","affiliation":[{"name":"Department of Dental Pharmacology School of Dentistry Jeonbuk National University Jeonju 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