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Beta\u2010blockers are known to inhibit cell progression. The aim of this study is to explain the mechanism of action of \u03b2\u2010adrenergic receptors agonists and antagonists on apoptosis in chronic myeloid leukaemia cells. We tried to determine the effect of combined treatment of \u03b2\u2010adrenergic and cholinergic drugs on adrenergic \u03b2<jats:sub>1<\/jats:sub> and \u03b2<jats:sub>2<\/jats:sub> gene expression, cell proliferation and apoptosis in chronic myeloid leukaemia K562 cells. Cell proliferation was evaluated by the 5\u2010bromo\u20102\u2010deoxy\u2010uridine (BrdU) incorporation kit. Caspase 3, 8, 9 activities were measured by the caspase assay kit. Protein expression level was detected by western blotting. We found that exposure to propranolol either by combination with carbachol facilitates additive effects on inhibition of caspase 3 and 8 expression in chronic myeloid leukaemia K562 cells. However, caspase 9 expression level was increased by propranolol alone or with propranolol and carbachol combination. The combined therapy of cholinergic and adrenergic receptor drugs will decrease cell proliferation in K562 cells. This decrease in cell proliferation may be mediated by the mitochondrial\u2010dependent intrinsic apoptosis pathway.<\/jats:p>","DOI":"10.1111\/1440-1681.13627","type":"journal-article","created":{"date-parts":[[2022,1,28]],"date-time":"2022-01-28T01:33:07Z","timestamp":1643333587000},"page":"515-524","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cross\u2010talk of cholinergic and \u03b2\u2010adrenergic receptor signalling in chronic myeloid leukemia K562 cells"],"prefix":"10.1111","volume":"49","author":[{"given":"Banu","family":"Ayd\u0131n","sequence":"first","affiliation":[{"name":"Department of Biophysics Marmara University School of Medicine Basic Medical Sciences Building  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehmet Zafer","family":"G\u00f6ren","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology Marmara University School of Medicine Basic Medical Sciences Building  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zehra","family":"Kanl\u0131","sequence":"additional","affiliation":[{"name":"Department of Biophysics Marmara University School of Medicine Basic Medical Sciences Building  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5757-2198","authenticated-orcid":false,"given":"H\u00fclya","family":"Cabadak","sequence":"additional","affiliation":[{"name":"Department of Biophysics Marmara University School of Medicine Basic Medical Sciences Building  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,2,14]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_10_2_1","DOI":"10.1042\/BJ20031705"},{"doi-asserted-by":"publisher","key":"e_1_2_10_3_1","DOI":"10.1152\/ajpcell.00514.2008"},{"doi-asserted-by":"publisher","key":"e_1_2_10_4_1","DOI":"10.1016\/S0163-7258(99)00071-6"},{"doi-asserted-by":"publisher","key":"e_1_2_10_5_1","DOI":"10.1254\/jphs.FM0070109"},{"doi-asserted-by":"publisher","key":"e_1_2_10_6_1","DOI":"10.1016\/S0165-6147(03)00195-0"},{"doi-asserted-by":"publisher","key":"e_1_2_10_7_1","DOI":"10.1038\/bjp.2008.185"},{"doi-asserted-by":"publisher","key":"e_1_2_10_8_1","DOI":"10.2174\/1871520618666180406123154"},{"doi-asserted-by":"publisher","key":"e_1_2_10_9_1","DOI":"10.3109\/10799893.2010.506484"},{"doi-asserted-by":"publisher","key":"e_1_2_10_10_1","DOI":"10.1074\/jbc.M211670200"},{"doi-asserted-by":"publisher","key":"e_1_2_10_11_1","DOI":"10.1158\/1078-0432.CCR-11-0641"},{"doi-asserted-by":"publisher","key":"e_1_2_10_12_1","DOI":"10.1007\/s10549-010-0822-2"},{"doi-asserted-by":"publisher","key":"e_1_2_10_13_1","DOI":"10.1158\/0008-5472.CAN-10-0522"},{"doi-asserted-by":"publisher","key":"e_1_2_10_14_1","DOI":"10.1172\/JCI40802"},{"doi-asserted-by":"publisher","key":"e_1_2_10_15_1","DOI":"10.1177\/1758834012439738"},{"doi-asserted-by":"publisher","key":"e_1_2_10_16_1","DOI":"10.1038\/sj.bjp.0704622"},{"doi-asserted-by":"publisher","key":"e_1_2_10_17_1","DOI":"10.1007\/s00210-003-0860-y"},{"doi-asserted-by":"publisher","key":"e_1_2_10_18_1","DOI":"10.1126\/science.1109237"},{"doi-asserted-by":"publisher","key":"e_1_2_10_19_1","DOI":"10.1016\/j.ejphar.2011.04.055"},{"doi-asserted-by":"publisher","key":"e_1_2_10_20_1","DOI":"10.1097\/MPA.0b013e318184f50c"},{"doi-asserted-by":"publisher","key":"e_1_2_10_21_1","DOI":"10.3727\/096504010X12828372551867"},{"doi-asserted-by":"publisher","key":"e_1_2_10_22_1","DOI":"10.18632\/oncotarget.1083"},{"doi-asserted-by":"publisher","key":"e_1_2_10_23_1","DOI":"10.1038\/bjc.1972.33"},{"doi-asserted-by":"publisher","key":"e_1_2_10_24_1","DOI":"10.1083\/jcb.144.2.281"},{"doi-asserted-by":"publisher","key":"e_1_2_10_25_1","DOI":"10.3390\/ijms14022334"},{"doi-asserted-by":"publisher","key":"e_1_2_10_26_1","DOI":"10.1007\/s00262-003-0474-8"},{"issue":"6","key":"e_1_2_10_27_1","first-page":"832","article-title":"Overexpression of caspase\u20109 triggers its activation and apoptosis in vitro","volume":"47","author":"Druskovic M","year":"2006","journal-title":"Croat Med J"},{"doi-asserted-by":"publisher","key":"e_1_2_10_28_1","DOI":"10.1038\/374421a0"},{"doi-asserted-by":"publisher","key":"e_1_2_10_29_1","DOI":"10.1152\/physrev.00021.2003"},{"doi-asserted-by":"publisher","key":"e_1_2_10_30_1","DOI":"10.1182\/blood.V45.3.321.321"},{"doi-asserted-by":"publisher","key":"e_1_2_10_31_1","DOI":"10.1016\/j.leukres.2013.05.018"},{"doi-asserted-by":"publisher","key":"e_1_2_10_32_1","DOI":"10.5472\/marumj.518983"},{"unstructured":"Ayd\u0131nB Kanl\u0131Z G\u00f6renZ CabadakH.The effect of beta adrenergic agonist and antagonists on caspase 3 caspase 8 and caspase 9 expression on K562 chronic myeloid leukemia cells. 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