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Given that the mammalian target of rapamycin (mTOR) signalling confers resistance to cancer therapy, we investigated whether mTOR contributes to radio\u2010resistance in NPC and pharmacological inhibition of mTOR can overcome radio\u2010resistance. We found that mTOR mRNA and protein levels, and phosphorylation of its downstream effector were increased in radio\u2010resistant NPC compared with parental cells. mTOR inhibitor temsirolimus inhibits proliferation and induces apoptosis in a panel of NPC cell lines. Importantly, temsirolimus acts synergistically with radiation and is effective against radio\u2010resistant cells. Using radio\u2010resistant xenograft mouse model, we validated the efficacy of temsirolimus in preventing tumour formation and inhibiting tumour growth. Temsirolimus overcome radio\u2010resistance in NPC via inhibiting mTOR signalling. Our work provides the pre\u2010clinical evidence that the combination of radiation and mTOR inhibitor may be a therapeutic strategy in NPC. Our findings might accelerate the initiation of clinical trials on radio\u2010resistant NPC patients using temsirolimus.<\/jats:p>","DOI":"10.1111\/1440-1681.13649","type":"journal-article","created":{"date-parts":[[2022,4,30]],"date-time":"2022-04-30T08:52:48Z","timestamp":1651308768000},"page":"703-709","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Inhibition of <scp>mTOR<\/scp> by temsirolimus overcomes radio\u2010resistance in nasopharyngeal carcinoma"],"prefix":"10.1111","volume":"49","author":[{"given":"Shiyong","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology &amp; Head and Neck Surgery, Xiangyang Central Hospital Affiliated Hospital of Hubei University of Arts and Science  Xiangyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Oncology, Xiangyang Central Hospital Affiliated Hospital of Hubei University of Arts and Science  Xiangyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Oncology, Xiangyang Central Hospital Affiliated Hospital of Hubei University of Arts and Science  Xiangyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangping","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology &amp; 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