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Because vascular tone is highly dependent on the cytosolic calcium concentration ([Ca<jats:sup>2+<\/jats:sup>]c), the current study aimed to investigate the effects of RuBPY on the intracellular mobilization of calcium stores of rat aortic vascular smooth muscle cells. Vascular reactivity experiments were performed in isolated aortic rings that were contracted with a high concentration of KCl or phenylephrine (Phe). Moreover, primary cultured vascular smooth muscle cells were used to measure [Ca<jats:sup>2+<\/jats:sup>]c by confocal microscopy. The NO donor RuBPY decreased the [Ca<jats:sup>2+<\/jats:sup>]c and reduced KCl and Phe\u2010induced contractile responses. The selective inhibitor of sarco\u2010endoplasmic Ca\u2010ATPase (SERCA) with thapsigargin impaired the effect of RuBPY on Phe\u2010induced contractile response. RuBPY also reduced caffeine\u2010induced contraction, and the contraction dependent on the capacitive Ca<jats:sup>2+<\/jats:sup> influx. Therefore, our results suggest that NO released from RuBPY decreased [Ca<jats:sup>2+<\/jats:sup>]c by calcium influx blockade and activation of guanylyl\u2010cyclase\u2010cGMP\u2010GK pathway. These results indicate that RuBPY increases Ca<jats:sup>2+<\/jats:sup> storage in the sarcoplasmic reticulum by SERCA activation and also by capacitive Ca<jats:sup>2+<\/jats:sup> influx inhibition, which is dependent on the intracellular release of nitric oxide from this compound.<\/jats:p>","DOI":"10.1111\/1440-1681.13652","type":"journal-article","created":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T07:29:35Z","timestamp":1652081375000},"page":"759-766","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>RuBPY<\/scp> decreases intracellular calcium by decreasing influx and increasing storage"],"prefix":"10.1111","volume":"49","author":[{"given":"Amanda de Carvalho","family":"Pereira","sequence":"first","affiliation":[{"name":"Faculty of Medicine of Itajub\u00e1 (FMIt)  Itajub\u00e1 MInas Gerais 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