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Kinesin family member 23 (KIF23) is involved in cytokinesis and associated with autophagy. The role of KIF23 in autophagy\u2010induced imatinib resistance in CML was investigated. First, to induce drug resistance, CML cells were exposed to increasing concentrations of imatinib. The concentration of imatinib resistance in CML cells was screened through upregulation of 50% inhibitory concentration (IC<jats:sub>50<\/jats:sub>) values. KIF23 was elevated in imatinib\u2010resistant tissues and cells of CML. Second, knockdown of KIF23 reduced IC<jats:sub>50<\/jats:sub> values of imatinib\u2010resistant CML cells to imatinib. Moreover, silence of KIF23 also suppressed cell proliferation and promoted apoptosis of imatinib\u2010resistant CML cells. Third, immunofluorescence analysis showed that the number of LC3 bright spots in imatinib\u2010resistant CML cells was reduced by silence of KIF23. Knockdown of KIF23 upregulated p62 expression and downregulated the expression ratio of LC3\u2010II to LC3\u2010I in imatinib\u2010resistant CML cells. Last, silence of KIF23 decreased nuclear \u03b2\u2010catenin and increased cytoplasmic \u03b2\u2010catenin in imatinib\u2010resistant CML cells. Activator of Wnt\/\u03b2\u2010catenin attenuated KIF23 silence\u2010induced increase of apoptosis and decrease of autophagy in imatinib\u2010resistant CML cells. In conclusion, loss of KIF23 repressed autophagy\u2010induced imatinib resistance in CML cells through inactivation of Wnt\/\u03b2\u2010catenin pathway.<\/jats:p>","DOI":"10.1111\/1440-1681.13718","type":"journal-article","created":{"date-parts":[[2022,9,6]],"date-time":"2022-09-06T14:04:33Z","timestamp":1662473073000},"page":"1334-1341","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["<scp>KIF23<\/scp> promotes autophagy\u2010induced imatinib resistance in chronic myeloid leukaemia through activating Wnt\/\u03b2\u2010catenin pathway"],"prefix":"10.1111","volume":"49","author":[{"given":"Yong","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Pharmacy Hospital of Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy 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