{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:47:05Z","timestamp":1791362825253,"version":"4.3.1"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T00:00:00Z","timestamp":1678665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In paediatric patients, there is no data on the recommended area under the concentration\u2013time curve from 0 to 12\u2009h (AUC<jats:sub>0\u221212<\/jats:sub>) for free mycophenolic acid (fMPA), which is the active form of the drug, responsible for the pharmacological effect. We decided to establish the limited sampling strategy (LSS) for fMPA for its use in MPA therapeutic monitoring in children with nephrotic syndrome treated with mycophenolate mofetil (MMF). This study included 23 children (aged 11\u2009\u00b1\u20094\u2009years) from whom eight blood samples were collected within 12\u2009h after MMF administration. The fMPA was determined using the high\u2010performance liquid chromatography with fluorescence detection method. LSSs were estimated with the use of R software and bootstrap procedure. The best model was chosen based on a number of profiles with AUC predicted within \u00b1\u200920% of AUC<jats:sub>0\u221212<\/jats:sub> (good guess), <jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>, mean prediction error (%MPE) of \u00b110% and mean absolute error (%MAE) of less than 25%. The fMPA AUC<jats:sub>0\u221212<\/jats:sub> was 0.1669\u2009\u00b1\u20090.0697\u2009\u03bcg\u2009h\/mL and the free fraction was within 0.16%\u20130.81%. In total, there were 92 equations developed of which five fulfilled the acceptance criteria for %MPE, %MAE, good guess &gt;80% and <jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u2009&gt;\u20090.900. These equations consisted of three time points: model 1 (C<jats:sub>1<\/jats:sub>, C<jats:sub>2<\/jats:sub>, C<jats:sub>6<\/jats:sub>), model 2 (C<jats:sub>1<\/jats:sub>, C<jats:sub>3<\/jats:sub>, C<jats:sub>6<\/jats:sub>), model 3 (C<jats:sub>1<\/jats:sub>, C<jats:sub>4<\/jats:sub>, C<jats:sub>6<\/jats:sub>), model 5 (C<jats:sub>0<\/jats:sub>, C<jats:sub>1<\/jats:sub>, C<jats:sub>2<\/jats:sub>), and model 6 (C<jats:sub>1<\/jats:sub>, C<jats:sub>2<\/jats:sub>, C<jats:sub>9<\/jats:sub>). Although blood sampling up to 9\u00a0h after MMF dosing is impractical, it is crucial to include C<jats:sub>6<\/jats:sub> or C<jats:sub>9<\/jats:sub> in LSS to assess fMPA AUC<jats:sub>pred<\/jats:sub> correctly. The most practical fMPA LSS, which fulfilled the acceptance criteria in the estimation group, was fMPA AUC<jats:sub>pred<\/jats:sub>\u00a0=\u00a00.040\u2009+\u20092.220\u2009\u00d7\u2009C<jats:sub>0<\/jats:sub>\u2009+\u20091.130\u2009\u00d7\u2009C<jats:sub>1<\/jats:sub>\u2009+\u20091.742\u2009\u00d7\u2009C<jats:sub>2<\/jats:sub>. Further studies should define the recommended fMPA AUC<jats:sub>0\u221212<\/jats:sub> value in children with nephrotic syndrome.<\/jats:p>","DOI":"10.1111\/1440-1681.13765","type":"journal-article","created":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T08:44:59Z","timestamp":1677487499000},"page":"486-496","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Limited sampling strategy to predict free mycophenolic acid area under the concentration\u2013time curve in paediatric patients with nephrotic syndrome"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2764-1575","authenticated-orcid":false,"given":"Joanna","family":"Sobiak","sequence":"first","affiliation":[{"name":"Department of Physical Pharmacy and Pharmacokinetics Poznan University of Medical Sciences  Pozna\u0144 Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2707-5956","authenticated-orcid":false,"given":"Pawe\u0142","family":"\u017bero","sequence":"additional","affiliation":[{"name":"Department of Drug Chemistry Medical University of Warsaw  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7365-553X","authenticated-orcid":false,"given":"Jacek","family":"Zachwieja","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology and Hypertension Poznan University of Medical Sciences  Pozna\u0144 Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1758-8587","authenticated-orcid":false,"given":"Danuta","family":"Ostalska\u2010Nowicka","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology and Hypertension Poznan University of Medical Sciences  Pozna\u0144 Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9110-4312","authenticated-orcid":false,"given":"Tomasz","family":"Pawi\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Drug Chemistry Medical University of Warsaw  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-200746010-00002"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00228-022-03295-1"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.5114\/aoms.2011.26618"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00228-021-03265-z"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/09546634.2018.1468068"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0000000000000871"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0000000000000799"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-200746020-00005"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.2012.04305.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13318-021-00713-0"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcpt.13743"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/17425255.2021.1843633"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V133759"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0012.2001.150607.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2018.01.040"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-200408000-00017"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40261-019-00844-y"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00228-018-2599-x"},{"key":"e_1_2_9_20_1","first-page":"165","volume-title":"Adv Clin Chem","author":"Dasgupta A","year":"2016"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-021-02139-z"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0000000000000640"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40262-013-0124-z"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0000000000000258"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.00320116"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2015.06.017"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-014-1247-1"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/0091270009359526"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.2004.02235.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00228-006-0215-y"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2012.08.001"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ftd.0000211821.73231.8a"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26123723"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcp.14590"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0b013e3181f01397"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.09.036"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-002-1057-1"}],"container-title":["Clinical and Experimental Pharmacology and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1440-1681.13765","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1440-1681.13765","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1440-1681.13765","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T05:23:44Z","timestamp":1692681824000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1440-1681.13765"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,13]]},"references-count":36,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1111\/1440-1681.13765"],"URL":"https:\/\/doi.org\/10.1111\/1440-1681.13765","archive":["Portico"],"relation":{},"ISSN":["0305-1870","1440-1681"],"issn-type":[{"value":"0305-1870","type":"print"},{"value":"1440-1681","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,13]]},"assertion":[{"value":"2022-10-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}