{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:22:39Z","timestamp":1747182159946,"version":"3.40.5"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T00:00:00Z","timestamp":1682640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81971978"],"award-info":[{"award-number":["81971978"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We first reported the <jats:italic>vanM<\/jats:italic> vancomycin resistance gene in enterococci in Shanghai, China in 2006 and later found it to be the predominant <jats:italic>van<\/jats:italic> gene in vancomycin\u2010resistant enterococci (VRE). In this study, we successively collected 1292 <jats:italic>Enterococcus faecium<\/jats:italic> and <jats:italic>Enterococcus faecalis<\/jats:italic> strains from in\u2010 and outpatients at Huashan Hospital, Fudan University and found that nearly all of the isolates (1290\/1292) were vancomycin\u2010sensitive determined by the VITEK 2 system. However, using a modified macromethod\u2010based disk diffusion test, 10 <jats:italic>E. faecium<\/jats:italic> isolates that were previously determined to be vancomycin\u2010sensitive by the VITEK 2 system were found to have colonies in the vancomycin disk inhibition zone. Pulse\u2010field gel electrophoresis results showed that each randomly selected colony in the inhibition zone belonged to the same clone as the original strain. All 10 isolates were later found to be <jats:italic>vanM<\/jats:italic>\u2010positive. The disk diffusion\u2010based method may aid in the detection of <jats:italic>vanM<\/jats:italic>\u2010positive <jats:italic>E. faecium<\/jats:italic> with low vancomycin minimum inhibitory concentrations and prevent missing the detection of vancomycin sensitivity\u2010variable enterococci.<\/jats:p>","DOI":"10.1111\/1440-1681.13772","type":"journal-article","created":{"date-parts":[[2023,3,21]],"date-time":"2023-03-21T02:44:05Z","timestamp":1679366645000},"page":"554-560","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Disk diffusion\u2010based method aids in the detection of <i>vanM<\/i>\u2010positive <i>Enterococcus faecium<\/i> with low vancomycin <scp>minimum inhibitory concentrations<\/scp>"],"prefix":"10.1111","volume":"50","author":[{"given":"Jue","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Antibiotics, Huashan Hospital Fudan University  Shanghai China"},{"name":"Key Laboratory 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