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Rats were divided into five groups: control, cecal ligation and puncture (CLP), CLP\u2009+\u2009MEL, CLP\u2009+\u2009ASA and CLP\u2009+\u2009MEL\u2009+\u2009ASA. The effects of MEL (10\u2009mg\/kg), ASA (100\u2009mg\/kg) and their combination on oxidative stress, inflammation and histopathology were evaluated in septic rats\u2019 lung tissues. Sepsis\u2010induced oxidative stress and inflammation were evident through increased levels of malondialdehyde (MDA), myeloperoxidase (MPO), total oxidant status (TOS)\u00a0and oxidative stress index (OSI); decreased levels of superoxide dismutase (SOD), glutathione (GSH), catalase (CAT) and glutathione peroxidase (GPx); and elevated levels of tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) and interleukin\u20101 \u03b2 (IL\u20101\u03b2) in the lung tissue. Treatment with MEL, ASA and their combination significantly improved antioxidant capacity and reduced oxidative stress, with the combination treatment being more effective. The combination treatment also significantly reduced TNF\u2010\u03b1 and IL\u20101\u03b2 levels and improved peroxisome proliferator\u2010activated receptor (PPAR), arylesterase (ARE) and paraoxonase (PON) levels in the lung tissue. Histopathological examination showed reduced oedema and lymphocyte infiltration with a lung tissue appearance similar to the control group. Immunohistochemical staining for caspase 3 demonstrated reduced immune positivity in the treatment groups. In conclusion, this study supports the potential synergistic protective effects of MEL and ASA in treating sepsis\u2010induced lung injury. The combination therapy could effectively reduce oxidative stress, inflammation and improve antioxidant capacity in septic rats, suggesting a promising strategy for treating sepsis\u2010induced lung injury.<\/jats:p>","DOI":"10.1111\/1440-1681.13780","type":"journal-article","created":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T07:41:33Z","timestamp":1684395693000},"page":"634-646","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Protective effect of melatonin and ascorbic acid combination on sepsis\u2010induced lung injury: An Experimental study"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3140-0755","authenticated-orcid":false,"given":"Hilal","family":"\u00dcst\u00fcnda\u011f","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Erzincan Binali Y\u0131ld\u0131r\u0131m University  Erzincan 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