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Rosavin is the major bioactive ingredient of <jats:italic>Rhodiola Rosea<\/jats:italic> L. with multiple therapeutic effects. The purpose of this was to investigate the role of rosavin in I\/R\u2010induced cerebral injury. A cell oxygen\u2013glucose deprivation and reoxygenation (OGD\/R) model and a mouse middle cerebral artery occlusion (MCAO) model were established to induce I\/R injury in vitro and in vivo, respectively. MCAO\u2010treated mice and OGD\/R\u2010challenged human brain microvascular endothelial cells (HBMVECs) were administrated with or without rosavin at various concentrations. Rosavin\u2010treated mice showed reduced infarct volume, neuronal loss and neuronal cytotoxicity in I\/R\u2010injured brains. Rosavin treatment downregulated the expression of pro\u2010inflammatory cytokines, reduced apoptosis and inhibited the activation of nuclear factor \u03ba B in I\/R\u2010injured mice and HBMVECs. Administration with rosavin also alleviated mouse brain oedema and upregulated tight junction proteins in mouse brains after I\/R injury, suggesting that rosavin protected mice against I\/R\u2010induced BBB disruption. Further analysis revealed that rosavin reduced the BBB permeability in I\/R\u2010injured mice and HBMVECs by inhibiting autophagy. Moreover, rosavin intervention inhibited I\/R injury\u2010induced activation of the mitogen\u2010activated protein kinase (MAPK) pathway and upregulation of matrix metalloproteinases in both mouse and cell models. In conclusion, rosavin protects the BBB against I\/R injury possibly by regulating the MAPK pathway. The above results provide a rationale for further exploration of rosavin as a therapeutic candidate for cerebral I\/R injury.<\/jats:p>","DOI":"10.1111\/1440-1681.13781","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T09:55:47Z","timestamp":1685526947000},"page":"664-676","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Rosavin protects the blood\u2013brain barrier against ischemia\/reperfusion\u2010induced cerebral injury by regulating <scp>MAPK<\/scp>\u2010mediated <scp>MMPs<\/scp> pathway"],"prefix":"10.1111","volume":"50","author":[{"given":"Hongyun","family":"Zou","sequence":"first","affiliation":[{"name":"Department of Anesthesiology The First Affiliated Hospital of Anhui Medical University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology The First 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