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However, whether GA affects sepsis due to infections remains unknown. In this study, alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen and creatinine in serum; neutrophil gelatinase\u2010associated lipocalin and kidney injury molecule\u20101 in the urine, cytokines (tumour necrosis factor alpha, interleukin\u20101\u03b2 and interleukin\u20106) in the bronchoalveolar lavage fluid and myeloperoxidase in the lung tissues were measured using enzyme\u2010linked immunosorbent assay kits. Pathological injury was measured by hematoxylin and eosin staining and neutrophils were measured by flow cytometry analysis; related expressions were analysed by qPCR, western blot and immunofluorescence assay. The results showed that GA significantly ameliorated cecum ligation and puncture (CLP)\u2010triggered liver, kidney and lung injury in septic mice. In addition, we found that GA dose\u2010dependently inhibited microthrombosis and alleviated coagulopathy in septic mice. Further molecular mechanism analysis suggests that GA may act through upregulation of heat shock factor 1 and tissue\u2010type plasminogen activator. In conclusion, our study elucidated the protective effects of GA in a mouse model established using CLP, and the results reveal that GA may be a promising agent for sepsis.<\/jats:p>","DOI":"10.1111\/1440-1681.13798","type":"journal-article","created":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T02:12:35Z","timestamp":1686622355000},"page":"698-707","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Geldanamycin ameliorates multiple organ dysfunction and microthrombosis in septic mice by inhibiting the formation of the neutrophil extracellular network by activating heat shock factor 1 <scp>HSF1<\/scp>"],"prefix":"10.1111","volume":"50","author":[{"given":"Jing","family":"Li","sequence":"first","affiliation":[{"name":"Department of Critical Care Medicine, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital Third Hospital of Shanxi Medical University  Taiyuan 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