{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:10:57Z","timestamp":1762254657720,"version":"3.40.5"},"reference-count":28,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T00:00:00Z","timestamp":1695168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81803129"],"award-info":[{"award-number":["81803129"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["pericles.pericles-gcp.literatumonline.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We previously revealed the role of prolactin (PRL) in antibody production and disease activity in patients with systemic lupus erythematosus. In this study, we sought to determine whether inhibition of PRL could improve lupus\u2010like disease in MRL\/lpr mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression levels of PRL in various cell types of lupus patients were measured by flow cytometry. The effects of anti\u2010PRL on animal survival, renal histopathology, creatinine, proteinuria, anti\u2010dsDNA antibody, cytokine production, splenomegaly and lymphadenopathy were assessed. The effect of anti\u2010PRL on the Jak2\u2010Stat3 signalling pathway was detected by western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Prolactin was upregulated in B cells, neutrophils, CD4+ T cells, and monocytes isolated from patients with lupus. Furthermore, inhibition of PRL by anti\u2010PRL treatment around the time of onset prolonged the survival of MRL\/lpr mice, significantly reduced anti\u2010dsDNA antibody production, and alleviated symptoms of lupus nephritis, splenomegaly, and lymphadenopathy. In addition, anti\u2010PRL\u2010treated mice showed a decrease in the levels of pathogenic cytokines such as IL\u201021 and IL\u20106. Furthermore, mechanistically, anti\u2010PRL treatment significantly reduced the levels of p\u2010Jak2 and p\u2010Stat3 in MRL\/lpr mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In summary, these data suggest that PRL inhibition alleviates lupus\u2010like disease in MRL\/lpr mice by modulating the Jak2\u2010Stat3 signalling cascade. More importantly, our results imply the potential of PRL inhibitors and may provide a novel therapeutic approach for lupus.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1440-1681.13818","type":"journal-article","created":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T07:48:05Z","timestamp":1695196085000},"page":"936-943","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Anti\u2010prolactin treatment alleviates lupus conditions by regulating the <scp>JAK2<\/scp>\u2013<scp>STAT3<\/scp> pathway"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6568-1540","authenticated-orcid":false,"given":"Ying","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Dermatology, Zhongshan Hospital Fudan University  Shanghai People's Republic of 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