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Chronic lung failure after lung transplantation is mainly caused by bronchiolitis obliterans syndrome (BOS) and is mainly characterized by lung tissue fibrosis. Pulmonary epithelial\u2010mesenchymal transformation (EMT) is crucial for pulmonary fibrosis. Telocytes (TCs), a new type of mesenchymal cells, play a protective role in various acute injuries. For exploring the anti\u2010pulmonary fibrosis effect of TCs in the BOS model in vitro and the related mechanism, rat tracheal epithelial (RTE) cells were treated with transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2) to simulate lung tissue fibrosis in vitro. The RTE cells were then co\u2010cultured with TCs primarily extracted from rat lung tissue. Western blot, Seahorse XF Analysers and enzyme\u2010linked immunosorbent assay were used to detect the level of EMT and aerobic respiration of RTE cells. Furthermore, anti\u2010hepatocyte growth factor (anti\u2010HGF) antibody was exogenously added to the cultured cells to explore further mechanisms. Moreover, hexokinase 2 (HK2) in RTE cells was knocked down to assess whether it influences the blocking effect of the anti\u2010HGF antibody. TGF\u2010\u03b2 could induce lung tissue fibrosis in RTE cells in vitro. Nevertheless, TCs co\u2010culture decreased the level of EMT, glucose metabolic indicators (lactate and ATP) and oxygen levels. Furthermore, TCs released hepatocyte growth factor (HGF). Therefore, the exogenous addition of anti\u2010HGF antibody in the co\u2010culture system blocked the anti\u2010lung tissue fibrosis effect. However, HK2 knockdown attenuated the blocking effect of the anti\u2010HGF antibody. In conclusion, TCs can protect against lung tissue fibrosis by releasing HGF, a process dependent on HK2.<\/jats:p>","DOI":"10.1111\/1440-1681.13823","type":"journal-article","created":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T08:55:27Z","timestamp":1694854527000},"page":"964-972","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Telocytes protect against lung tissue fibrosis through hexokinase 2\u2010dependent pathway by secreting hepatocyte growth factor"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6187-4313","authenticated-orcid":false,"given":"Shaoyuan","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery, Zhongshan Hospital (Xiamen) Fudan University  Xiamen China"},{"name":"Department of Thoracic Surgery, Zhongshan Hospital Fudan University  Shanghai China"},{"name":"Cancer Center, Zhongshan Hospital Fudan University  Shanghai China"},{"name":"Key Laboratory of Lung Inflammation and Injury  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linyi","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Zhongshan Hospital Fudan University  Shanghai China"},{"name":"Cancer Center, Zhongshan Hospital Fudan University  Shanghai China"},{"name":"Key Laboratory of Lung Inflammation and Injury  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Borong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Zhongshan Hospital (Xiamen) Fudan University  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0218-2533","authenticated-orcid":false,"given":"Siyun","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Zhongshan Hospital Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianmin","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Zhongshan Hospital Fudan University  Shanghai China"},{"name":"Cancer Center, Zhongshan Hospital Fudan University  Shanghai China"},{"name":"Key Laboratory of Lung Inflammation and Injury  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0708-5556","authenticated-orcid":false,"given":"Lijie","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Zhongshan Hospital Fudan University  Shanghai China"},{"name":"Cancer Center, Zhongshan Hospital Fudan University  Shanghai China"},{"name":"Key Laboratory of Lung Inflammation and Injury  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Zhongshan Hospital (Xiamen) Fudan University  Xiamen China"},{"name":"Department of Thoracic Surgery, Zhongshan Hospital Fudan University  Shanghai China"},{"name":"Cancer Center, Zhongshan Hospital Fudan University  Shanghai China"},{"name":"Key Laboratory of Lung Inflammation and Injury  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201506-1063ST"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1204664"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.03.00039103"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-4975(95)00751-6"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201002-0211OC"},{"issue":"1","key":"e_1_2_10_7_1","first-page":"5","article-title":"Bronchiolitis obliterans syndrome\u2010free survival after lung transplantation: an International Society for Heart and Lung Transplantation thoracic transplant registry analysis. 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