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Therefore, the present study aimed to characterize the putative alterations in skeletal muscle mass in female rats subjected to a FM model by inducing chronic diffuse hyperalgesia (CDH) through double injections of acidic saline (pH\u20094.0) into the left gastrocnemius muscle at 5\u2010day intervals. To determine protein turnover, the total proteolysis, proteolytic system activities and protein synthesis were evaluated in oxidative soleus muscles of pH\u20097.2 (control) and pH\u20094.0 groups at 7\u2009days after CDH induction. All animals underwent behavioural analyses of mechanical hyperalgesia, strength and motor performance. Our results demonstrated that, in addition to hyperalgesia, rats injected with acidic saline exhibited skeletal muscle loss, as evidenced by a decrease in the soleus fibre cross\u2010sectional area. This muscle loss was associated with increased proteasomal proteolysis and expression of the atrophy\u2010related gene (muscle RING\u2010finger protein\u20101), as well as reduced protein synthesis and decreased protein kinase B\/S6 pathway activity. Although the plasma corticosterone concentration did not differ between the control and pH\u20094.0 groups, the removal of the adrenal glands attenuated hyperalgesia, but it did not prevent the increase in muscle protein loss in acidic saline\u2010injected animals. The data suggests that the stress\u2010related hypothalamic\u2013pituitary\u2013adrenal axis is involved in the development of hyperalgesia, but is not responsible for muscle atrophy observed in the FM model induced by intramuscular administration of acidic saline. Although the mechanisms involved in the attenuation of hyperalgesia in rats injected with acidic saline and subjected to adrenalectomy still need to be elucidated, the results found in this study suggest that glucocorticoids may not represent an effective therapeutic approach to alleviate FM symptoms.<\/jats:p>","DOI":"10.1111\/1440-1681.13837","type":"journal-article","created":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T05:49:20Z","timestamp":1704952160000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Adrenalectomy attenuates hyperalgesia but does not regulate muscle wasting in a female rat model of fibromyalgia"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9735-6190","authenticated-orcid":false,"given":"Daniely Messias","family":"Costa","sequence":"first","affiliation":[{"name":"Laboratory of Basic and Behavioral Neuroendocrinology (LANBAC), Department of 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