{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T02:28:16Z","timestamp":1787797696347,"version":"build-2784847793"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T00:00:00Z","timestamp":1712016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chronic stress often triggers gastrointestinal complications, including gastric injury and ulcers. Understanding the role of heat shock protein 27 (HSP27) in stress\u2010induced gastric ulcers could unveil novel therapeutic targets. Here, we established a stress\u2010induced gastric ulcer rat model using water immersion restraint stress and administered adenovirus\u2010packaged HSP27 overexpression vector. Gastric ulcer severity was scored, and mucosal changes were assessed. Gastric epithelial and endothelial cells were treated with lipopolysaccharide and transfected with HSP27 overexpression vectors to evaluate cell viability, migration and angiogenesis. Expression levels of HSP27, C\u2010X\u2010C motif chemokine ligand 12 (CXCL12) and C\u2010X\u2010C motif chemokine receptor 4 (CXCR4) were measured in tissues and cells. HSP27 expression was initially low during stress\u2010induced gastric ulceration but increased during ulcer healing. HSP27 overexpression accelerated ulcer healing in rats, promoting gastric epithelial cell proliferation and migration and gastric endothelial cell angiogenesis through the CXCL12\/CXCR4 axis. Inhibitor IT1t reversed the effects of HSP27 overexpression on cell proliferation, migration and angiogenesis. In summary, HSP27 overexpression facilitated ulcer healing, which was partially mediated by the CXCL12\/CXCR4 axis.<\/jats:p>","DOI":"10.1111\/1440-1681.13857","type":"journal-article","created":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T05:15:25Z","timestamp":1712121325000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Overexpression of <scp>HSP27<\/scp> accelerates stress\u2010induced gastric ulcer healing via the <scp>CXCL12<\/scp>\/<scp>CXCR4<\/scp> axis"],"prefix":"10.1111","volume":"51","author":[{"given":"Qiaoyan","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Digestive Endoscopy Xi'an International Medical Center Hospital  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Digestive Endoscopy Xi'an International Medical Center Hospital  China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.4291\/wjgp.v5.i1.40"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612823666161118144216"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-016-1305-6"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.13655"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.therap.2020.06.019"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10081964"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/iep.12410"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23112846"},{"issue":"7","key":"e_1_2_9_10_1","first-page":"5","article-title":"Role of heat shock proteins in gastric inflammation and ulcer healing","volume":"60","author":"Choi SR","year":"2009","journal-title":"J Physiol Pharmacol"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2014.12.019"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.20-11"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1815"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2005.02.005"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2018.10.004"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2016.06.006"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2019.11.007"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2013.01.009"},{"key":"e_1_2_9_19_1","first-page":"585","article-title":"Formation of new blood vessels during gastric ulcer healing. Role of bone marrow derived endothelial progenitor cells","volume":"68","author":"Ahluwalia A","year":"2017","journal-title":"J Physiol Pharmacol"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS103S450S"},{"key":"e_1_2_9_21_1","doi-asserted-by":"crossref","DOI":"10.17226\/25801","volume-title":"Guide for the Care and Use of Laboratory Animals","author":"Institute of Laboratory Animal Resources (US). Committee on Care, and Use of Laboratory Animals","year":"2011"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2012.1041"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/02770903.2023.2213332"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2018.05.003"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2011.06.030"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-70312-7"},{"key":"e_1_2_9_28_1","first-page":"11","article-title":"The protective effect of polysaccharide SAFP from Sarcodon aspratus on water immersion and restraint stress\u2010induced gastric ulcer and modulatory effects on gut microbiota Dysbiosis","volume":"11","author":"Zhang D","year":"2022","journal-title":"Foods"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2004.03484.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.03.009"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-020-06074-z"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.abc1044"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1159\/000475636"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejvs.2023.06.001"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-007-9946-6"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0145"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.5135"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19103217"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2013.01.019"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2016.05.025"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201900464RR"}],"container-title":["Clinical and Experimental Pharmacology and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1440-1681.13857","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T00:07:13Z","timestamp":1712880433000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1440-1681.13857"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,2]]},"references-count":40,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,5]]}},"alternative-id":["10.1111\/1440-1681.13857"],"URL":"https:\/\/doi.org\/10.1111\/1440-1681.13857","archive":["Portico"],"relation":{},"ISSN":["0305-1870","1440-1681"],"issn-type":[{"value":"0305-1870","type":"print"},{"value":"1440-1681","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,2]]},"assertion":[{"value":"2023-08-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13857"}}