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Pre\u2010osteoblastic MC3T3\u2010E1 cells were cultured in osteogenic medium and treated by Dex to mimic steroid\u2010induced osteonecrosis cellular model. They were then further transfected with control or AEBP1\u2010overexpressed lentiviral vectors. Finally, cells were treated with the PI3K inhibitor LY294002, with or without AEBP1\u2010overexpressed lentiviral vectors. AEBP1 expression showed a downward trend in MC3T3\u2010E1 cells under Dex treatment in a dose\u2010dependent manner. AEBP1\u2010overexpressed lentiviral vectors increased relative cell viability, alkaline phosphatase (ALP) staining, Alizarin red staining and osteoblastic differentiation markers including osteocalcin (OCN), osteopontin (OPN), collagen type I alpha 1 (COL1A1), runt\u2010related transcription factor 2 (RUNX2) and bone morphogenetic protein 2 (BMP2), but decreased cell apoptosis rate in MC3T3\u2010E1 cells under Dex treatment; besides, AEBP1\u2010overexpressed lentiviral vectors positively regulated p\u2010PI3K and p\u2010AKT expressions. Furthermore, LY294002 treatment decreased relative cell viability, Alizarin red staining, osteoblastic differentiation markers including OCN, OPN, RUNX2 and BMP, increased cell apoptosis rate and did not affect ALP staining in MC3T3\u2010E1 cells under Dex treatment; meanwhile, LY294002 treatment weakened the effect of AEBP1 overexpression vectors on the above cell functions. AEBP1 restores osteoblastic differentiation under Dex treatment by activating the PI3K\/AKT pathway.<\/jats:p>","DOI":"10.1111\/1440-1681.13923","type":"journal-article","created":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T04:09:58Z","timestamp":1727928598000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>AEBP1<\/scp> restores osteoblastic differentiation under dexamethasone treatment by activating <scp>PI3K<\/scp>\/<scp>AKT<\/scp> signalling"],"prefix":"10.1111","volume":"51","author":[{"given":"Rilong","family":"Jin","sequence":"first","affiliation":[{"name":"Center for Sport Medicine The First Affiliated Hospital, Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Sport Medicine The First Affiliated Hospital, Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yute","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Surgery The First Affiliated Hospital, Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Surgery The First Affiliated Hospital, Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jot.2019.12.004"},{"issue":"5","key":"e_1_2_8_3_1","volume":"6","author":"George G","year":"2022","journal-title":"J Am Acad Orthop Surg Glob Res 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