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The aim of this study is to explore the potential cardioprotective mechanism of NIB on MI injury caused by isoproterenol (ISO). We administered NIB to SD rats at 20 and 40\u2009mg\/kg daily for 7\u2009days in this study; this was followed by an ISO injection to induce MI injury. Parameters such as electrocardiogram readings, heart rate, serum concentrations of creatine kinase (CK) and creatine kinase\u2010MB (CK\u2010MB), levels of inflammatory markers, some histopathological assessments and oxidative stress markers were evaluated. We conducted Western blot analyses to evaluate protein expression related to apoptosis and the TLR4\u2013MyD88\u2010mediated NF\u2010\u03baB activation pathway. The L\u2010type Ca<jats:sup>2+<\/jats:sup> current (ICa\u2010L) and contraction of isolated ventricular cells from rats were identified using patch\u2010clamp methods and the IonOptix detection system. The treatment with NIB resulted in improvements in heart rate and ST\u2010segment changes, a reduction in CK and CK\u2010MB levels, the restoration of superoxide dismutase, catalase and glutathione levels and a decrease in malondialdehyde accumulation. Furthermore, NIB reduced the expression of inflammatory markers, lowered Ca<jats:sup>2+<\/jats:sup> levels and reactive oxygen species production and improved myocardial tissue morphology. It also countered ISO\u2010induced alterations in apoptosis and the TLR4\u2013MyD88\u2010dependent NF\u2010\u03baB activation pathway. Additionally, NIB considerably attenuated ICa\u2010L and reduced the contractile function of cardiomyocytes. These results suggest that NIB effectively mitigates ISO\u2010induced MI injury through anti\u2010inflammatory, antioxidative, and anti\u2010apoptotic mechanisms, potentially involving the TLR4\u2013MyD88\u2010dependent NF\u2010\u03baB activation pathway and calcium balance.<\/jats:p>","DOI":"10.1111\/1440-1681.70033","type":"journal-article","created":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T06:46:24Z","timestamp":1740984384000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Norisoboldine Alleviates Isoproterenol\u2010Induced Myocardial Ischemic Injury via the <scp>TLR4<\/scp>\u2013<scp>MyD88<\/scp>\u2010Dependent <scp>NF<\/scp>\u2010<scp>\u03baB<\/scp> Activation Pathway and Modulation of L\u2010Type Calcium Channels"],"prefix":"10.1111","volume":"52","author":[{"given":"Xin","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Integrative Chinese and Western Medicine Hebei University 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