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Pyroptosis in macrophages plays a pivotal role in lung tissue damage; however, its specific involvement in <jats:styled-content style=\"fixed-case\"><jats:italic>Mycobacterium abscessus<\/jats:italic><\/jats:styled-content> infection remains to be fully clarified. In this study, we identified that the rough morphotype of <jats:styled-content style=\"fixed-case\"><jats:italic>Mycobacterium abscessus<\/jats:italic><\/jats:styled-content> upregulates the ROS\/p65\/NLRP3\/GSDMD signalling pathway, thereby mediating pyroptosis in THP\u20101\u2010derived macrophages. This heightened ability to induce macrophage pyroptosis is attributed to the bacterium's capacity to sustain intracellular viability and proliferation. These findings offer valuable insights into the virulence mechanisms of <jats:styled-content style=\"fixed-case\"><jats:italic>Mycobacterium abscessus<\/jats:italic><\/jats:styled-content> and provide a foundation for future therapeutic interventions.<\/jats:p>","DOI":"10.1111\/1440-1681.70034","type":"journal-article","created":{"date-parts":[[2025,4,19]],"date-time":"2025-04-19T03:18:42Z","timestamp":1745032722000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Rough Morphotype of <scp><i>Mycobacterium abscessus<\/i><\/scp> Enhances Its Virulence Through <scp>ROS<\/scp>\/p65\/<scp>NLRP3<\/scp>\/<scp>GSDMD<\/scp>\u2010Mediated Macrophage Pyroptosis"],"prefix":"10.1111","volume":"52","author":[{"given":"Jingren","family":"Li","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Shanghai Pulmonary Hospital, School of Medicine, Tongji University  Shanghai 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