{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T18:30:47Z","timestamp":1788546647103,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sleep deprivation (SD) often disrupts normal sleep patterns, leading to anxiety\u2010like behaviours and cognitive impairments. Dexmedetomidine (DEX), a highly selective \u03b1\u20102 adrenergic agonist, has been reported to improve postoperative sleep quality and alleviate cognitive deficits. Evidence indicates that DEX can regulate the expression of histone deacetylase 5 (HDAC5), a substrate of the sleep\u2010regulating molecule salt\u2010inducible kinase 3 (SIK3). However, its specific role and the underlying mechanisms in regulating sleep structure remain unclear. This study aims to examine DEX's effects and potential mechanisms in regulating sleep structure in sleep\u2010deprived mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>C57BL\/6J mice were subjected to chronic sleep deprivation (CSD) for 20\u2009h (from 5 to 1\u2009PM the next day), followed by administration of DEX at 1 and 3\u2009PM daily for 7 consecutive days. Emotional behaviours were evaluated using the open\u2010field test. Sleep patterns were assessed during the recovery period using EEG\/EMG. Golgi\u2010Cox staining was used to determine neuronal dendritic damage. Western blotting was employed to detect the SIK3\/HDAC4\/5 pathway\u2010related protein levels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CSD disrupted the sleep structure of mice during the recovery period and induced anxiety\u2010like behaviour and neuronal dendritic damage. DEX administration alleviated the above effects caused by CSD. Mechanistically, DEX activated SIK3\/HDAC4\/5 signalling in CSD mice, while inhibition of SIK3 with a specific inhibitor attenuated DEX's effects on the pathway activation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>DEX can alleviate sleep rhythm disturbances caused by CSD in mice possibly via modulation of the SIK3\/HDAC4\/5 pathway, thereby improving anxiety\u2010like behaviours and cognitive function.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1440-1681.70086","type":"journal-article","created":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T00:39:27Z","timestamp":1763771967000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dexmedetomidine Alleviates Sleep Rhythm Abnormalities in Chronic Sleep\u2010Deprived Mice via Modulation of\n                    <scp>SIK3<\/scp>\n                    \/\n                    <scp>HDAC4<\/scp>\n                    \/5 Signalling"],"prefix":"10.1111","volume":"53","author":[{"given":"Zhengye","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Anesthesiology The Affiliated Changzhou Second People's Hospital of Nanjing Medical University  Changzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yitong","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The Affiliated Changzhou Second People's Hospital of Nanjing Medical University  Changzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The Affiliated Changzhou Second People's Hospital of Nanjing Medical University  Changzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhouquan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The Affiliated Changzhou Second People's Hospital of Nanjing Medical University  Changzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-024-10214-6"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-00188-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsr.13982"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/healthcare7010001"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/2377960820930209"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cnc.2021.01.008"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41105-022-00439-9"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-42758-y"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2024.116329"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-015-0419-5"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0000000000003567"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41105-023-00450-8"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/brainsci13071058"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/sleep\/zsae279"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05450-1"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05510-6"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43556-023-00128-0"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)02248-9"},{"issue":"1","key":"e_1_2_8_20_1","first-page":"533","article-title":"Dexmedetomidine Attenuates the Toxicity of \u03b2\u2010Amyloid on Neurons and Astrocytes by Increasing BDNF Production Under the Regulation of HDAC2 and HDAC5","volume":"19","author":"Wang Y.","year":"2019","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12158"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0000000000003347"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2024.10.279"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201643686"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsr.14062"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0000000000000361"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-022-01565-z"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-023-00823-1"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2022.175149"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1167312"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.15474"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.965661"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2024.115006"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-023-00776-5"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00019.2023"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2023.109710"}],"container-title":["Clinical and Experimental Pharmacology and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1440-1681.70086","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1440-1681.70086","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1440-1681.70086","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T02:39:36Z","timestamp":1768790376000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1440-1681.70086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,21]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1111\/1440-1681.70086"],"URL":"https:\/\/doi.org\/10.1111\/1440-1681.70086","archive":["Portico"],"relation":{},"ISSN":["0305-1870","1440-1681"],"issn-type":[{"value":"0305-1870","type":"print"},{"value":"1440-1681","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,21]]},"assertion":[{"value":"2025-03-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70086"}}