{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T18:10:10Z","timestamp":1768846210118,"version":"3.49.0"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018625","name":"Science and Technology Innovation Plan Of Shanghai Science and Technology Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018625","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Nontuberculous mycobacteria (NTM) infections, especially those caused by\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Mycobacterium abscessus<\/jats:italic>\n                    <\/jats:styled-content>\n                    , are extremely difficult to treat due to the organisms' intrinsic resistance to many antimicrobials. Myxin is a naturally occurring phenazine\u20105,10\u2010dioxide antibiotic from\n                    <jats:italic>Sorangium<\/jats:italic>\n                    sp., notable for its broad\u2010spectrum antimicrobial activity. In this study, we determined the susceptibilities of Myxin against 11 NTM reference strains and 194 clinical\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. abscessus<\/jats:italic>\n                    <\/jats:styled-content>\n                    isolates using microdilution assays. Additionally, time\u2010kill kinetic experiments were performed to distinguish the clearing efficiency of Myxin in broth. We also evaluated the efficacy of Myxin against intracellular\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. abscessus<\/jats:italic>\n                    <\/jats:styled-content>\n                    in THP\u20101 macrophages. Checkerboard assays assessed the interactions between Myxin and eight clinically important anti\u2010NTM antibiotics. Myxin demonstrated potent activity against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. abscessus<\/jats:italic>\n                    <\/jats:styled-content>\n                    with low MIC values (MIC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u20090.125\u2009mg\/L and MIC\n                    <jats:sub>90<\/jats:sub>\n                    \u2009=\u20090.5\u2009mg\/L). Treatment with 20\u2009\u00d7\u2009MIC of Myxin in 7H9 broth resulted in up to 10 log\n                    <jats:sub>10<\/jats:sub>\n                    CFU\/mL reduction after 4\u2009days of incubation. An intracellular concentration\u2010dependent bactericidal effect of Myxin was observed in THP\u20101 macrophages. Myxin exhibited no antagonism in combination with standard anti\u2010NTM drugs. As such, Myxin represents a promising candidate for the treatment of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. abscessus<\/jats:italic>\n                    <\/jats:styled-content>\n                    and other NTM infections.\n                  <\/jats:p>","DOI":"10.1111\/1440-1681.70091","type":"journal-article","created":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T00:49:27Z","timestamp":1763772567000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vitro Antibacterial Activity of Myxin Against\n                    <scp>\n                      <i>Mycobacterium abscessus<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5010-2298","authenticated-orcid":false,"given":"Zihao","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine Tongji University  Shanghai China"},{"name":"School of Medicine Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyu","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine Tongji University  Shanghai China"},{"name":"School of Medicine Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2965-9901","authenticated-orcid":false,"given":"Anqi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine Tongji University  Shanghai China"},{"name":"School of Medicine Tongji University  Shanghai 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Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yani","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine Tongji University  Shanghai China"},{"name":"School of Medicine Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"li","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine Tongji University  Shanghai China"},{"name":"School of Medicine Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuheng","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine Tongji University  Shanghai China"},{"name":"School of Medicine Tongji 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