{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T04:22:15Z","timestamp":1773807735836,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.620568\/2021\u201000"],"award-info":[{"award-number":["88887.620568\/2021\u201000"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["309464\/2022\u20106"],"award-info":[{"award-number":["309464\/2022\u20106"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2023\/15350\u20109"],"award-info":[{"award-number":["2023\/15350\u20109"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Acepromazine (ACP) is one of the most used sedatives in veterinary medicine, and its cardiovascular effects are well documented. In contrast, its effects on the respiratory system remain less well understood, particularly in the context of assessment of respiratory mechanics in murine models of asthma, where its use has become more common in recent years. This study aims to investigate the effect of ACP on respiratory mechanics in BALB\/c mice, specifically in an ovalbumin (OVA)\u2010induced model of allergic asthma. Mice received ACP (2.5\u2009mg\/kg) in combination with standard ketamine (100\u2009mg\/kg) and xylazine (10\u2009mg\/kg) anaesthesia. Respiratory mechanics were assessed in\u00a0vivo using forced oscillation technique (FOT) following methacholine (MCh) challenge. Additionally, tracheal rings and lung strips were evaluated in\u00a0vitro for airway smooth muscle (ASM) responsiveness to MCh. ACP administration significantly reduced Newtonian resistance (Rn) in OVA\u2010sensitised mice following MCh challenge, suggesting an inhibitory effect on ASM contractility. Furthermore, ACP mitigated increases in tissue damping (G) and hysteresivity (\n                    <jats:italic>\u03b7<\/jats:italic>\n                    ), particularly in the inflamed lungs of OVA\u2010treated mice. In\u00a0vitro, ACP induced a rightward shift in MCh concentration\u2013response curves in non\u2010inflamed ASM and lung strips. These findings highlight the potential confounding effects of ACP in studies assessing airway hyperresponsiveness and offer insights into how sedative choice can shape the scientific validity of studies involving assessment of respiratory mechanics.\n                  <\/jats:p>","DOI":"10.1111\/1440-1681.70112","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:58:09Z","timestamp":1773003489000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Acepromazine Reduces Airway Contraction in a Murine Model of Asthma"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6173-5428","authenticated-orcid":false,"given":"Alembert","family":"Lino\u2010Alvarado","sequence":"first","affiliation":[{"name":"Biomedical Engineering Laboratory, Escola Polit\u00e9cnica University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Aparecida","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Institute of Biomedical Science University of Sao Paulo  Sao Paulo Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen Silvia Moreno","family":"Serra","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Institute of Biomedical Science University of Sao Paulo  Sao Paulo Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabella Victoria Santana","family":"Melhado","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Institute of Biomedical Science University of Sao Paulo  Sao Paulo Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"Campos","sequence":"additional","affiliation":[{"name":"Unit of Pulmonary Pharmacology, School of Cancer and Pharmaceutical Sciences King's College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2137-9430","authenticated-orcid":false,"given":"Yanira","family":"Riffo\u2010Vasquez","sequence":"additional","affiliation":[{"name":"Unit of Pulmonary Pharmacology, School of Cancer and Pharmaceutical Sciences King's College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrique Takachi","family":"Moriya","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Laboratory, Escola Polit\u00e9cnica University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1956.8.6.587"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1992.72.1.168"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00706.2002"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535370221993095"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00243.2017"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-024-01991-5"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-5-15"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2021.103705"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani9100754"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/awf.2024.39"},{"issue":"6","key":"e_1_2_10_12_1","first-page":"475","article-title":"Perioperative Use of Selective Alpha\u20102 Agonists and Antagonists in Small Animals","volume":"45","author":"Lemke K. 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