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Bombesin receptor\u2010activated protein (BRAP), encoded by the human\n                    <jats:italic>C6orf89<\/jats:italic>\n                    gene, shares 83% identity with its mouse homologue encoded by\n                    <jats:italic>BC004004<\/jats:italic>\n                    . Both proteins have been identified in bronchial epithelial cells, and our previous studies found that BRAP overexpression alters the biological behaviour of cultured human bronchial epithelial cells, suggesting a potential role in regulating immune homeostasis in the respiratory tract. In this study, we used BC004004 knockout (\n                    <jats:italic>BC004004<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    ) mice, which lack expression of the BRAP homologue, to establish ovalbumin (OVA)\u2010induced or house dust mite (HDM)\u2010induced asthma models. Following OVA or HDM challenge,\n                    <jats:italic>BC004004<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice exhibited exacerbated airway inflammation compared with wild\u2010type controls, characterised by increased mucus production in the airway lumen, enhanced shedding of bronchial epithelial cells, and more pronounced infiltration of inflammatory cells surrounding the bronchi and bronchioles. Meanwhile, the levels of Th2 cytokines IL\u20104, IL\u20105, and IL\u201013 in the bronchoalveolar lavage fluid (BALF) were significantly elevated in\n                    <jats:italic>BC004004<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice after allergen exposure. In addition, serum levels of thymic stromal lymphopoietin (TSLP), a key epithelial\u2010derived mediator of type 2 immune responses, were increased in\n                    <jats:italic>BC004004<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice following OVA or HDM challenge. In cultured immortalised human bronchial epithelial cells, TSLP release was upregulated by BRAP knockdown and suppressed by BRAP overexpression. Further analysis revealed that BRAP homologue deficiency resulted in reduced E\u2010cadherin expression in asthmatic lung tissues. Together with the enhanced epithelial shedding observed in the lungs of allergen\u2010challenged\n                    <jats:italic>BC004004<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice, these findings suggest that loss of BRAP homologue compromises epithelial barrier integrity. The enhanced disruption of epithelial barrier integrity in\n                    <jats:italic>BC004004<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice may promote increased TSLP release from airway epithelial cells, thereby contributing to the exacerbation of allergic inflammation and asthma pathogenesis. Collectively, these results indicate a role for BRAP in maintaining epithelial barrier function during allergic asthma.\n                  <\/jats:p>","DOI":"10.1111\/1440-1681.70119","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T14:09:58Z","timestamp":1775052598000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>BRAP<\/scp>\n                    Homologue Deficiency Exacerbates Airway Inflammation in Mouse Models of Experimental Asthma\u2010Like Disease"],"prefix":"10.1111","volume":"53","author":[{"given":"Hui","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Physiology, Xiangya School of Basic Medical Science Central South University  Changsha Hunan China"},{"name":"Guangdong Provincial Key Laboratory of Tumor Interventional Diagnosis and Treatment Zhuhai Institute of Translational Medicine, Zhuhai People's Hospital Affiliated With Jinan University, Jinan University  Zhuhai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rujiao","family":"Liu","sequence":"additional","affiliation":[{"name":"Hunan Vocational College of Electronic and Technology  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenrui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Physiology, Xiangya School of Basic Medical Science Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Physiology, Xiangya School of Basic Medical Science Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaoyun","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Physiology, Xiangya School of Basic Medical Science Central South University  Changsha Hunan China"},{"name":"Department of Pathology, the Second Xiangya Hospital Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horst Christian","family":"Weber","sequence":"additional","affiliation":[{"name":"Section of Gastroenterology, and Department of Pathology and Laboratory Medicine Boston University School of Medicine  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangping","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Physiology, Xiangya School of Basic Medical Science Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,1]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.03.004"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.06.009"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050199"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI124606"},{"issue":"3","key":"e_1_2_10_6_1","first-page":"321","article-title":"A Thymic Stromal Cell Line Supports in Vitro Development of Surface IgM+ B Cells and Produces a Novel Growth Factor Affecting B and T Lineage Cells","volume":"22","author":"Friend S. 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