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Electroacupuncture (EA) may confer neuroprotection by modulating key cellular processes; however, its precise role in regulating autophagy\u2010ferroptosis crosstalk remains largely unclear. The present study aimed to investigate the neuroprotective potential of EA in cerebral ischemia\u2013reperfusion injury, with a focus on exploring potential pathways involving autophagy and ferroptosis regulation. Focal cerebral ischemia\u2013reperfusion injury was modelled using middle cerebral artery occlusion\/reperfusion (MCAO\/R) in\u00a0vivo and oxygen\u2013glucose deprivation\/reperfusion (OGD\/R) in\u00a0vitro. The therapeutic effect of EA on MCAO\/R mice was assessed using several methods, including behavioural tests, cerebral blood flow measurement, and cerebral infarction volume analysis. Molecular analyses used immunofluorescence staining, western blot analysis, and transmission electron microscopy to examine signal transducer and activator of transcription 3 (STAT3)\/hypoxia\u2010inducible factor\u20101\u03b1 (HIF\u20101\u03b1) pathway activity and its relationship with autophagy\/ferroptosis markers. In MCAO\/R mice, EA intervention improved neurological functional recovery, decreased cerebral infarction, and enhanced blood flow. EA also downregulated activation of the STAT3\/HIF\u20101\u03b1 signalling pathway. Furthermore, EA was associated with reduced markers of excessive autophagy and reduced ferroptosis markers in neurons. IL\u20106\u2010mediated enhancement of STAT3 phosphorylation significantly weakened EA's protective effects against cerebral ischemia\u2013reperfusion injury. In\u00a0vitro, STAT3 knockdown prevented OGD\/R\u2010induced activation of STAT3\/HIF\u20101\u03b1 signalling and was accompanied by reduced autophagy and ferroptosis markers. Our results suggest that EA exerts neuroprotective effects against cerebral ischemic injury, which may be associated with modulation of autophagy and ferroptosis markers via the STAT3\/HIF\u20101\u03b1 signalling pathway.<\/jats:p>","DOI":"10.1111\/1440-1681.70146","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T10:51:11Z","timestamp":1783421471000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Electroacupuncture Alleviates Focal Cerebral Ischemia\u2013Reperfusion Injury and Is Associated With Modulation of Autophagy\u2010Ferroptosis Involving the\n                    <scp>STAT3<\/scp>\n                    \/\n                    <scp>HIF<\/scp>\n                    \u20101\u03b1 Signalling Pathway"],"prefix":"10.1111","volume":"53","author":[{"given":"Sisi","family":"Li","sequence":"first","affiliation":[{"name":"Rehabilitation Medicine Center The Second Affiliated Hospital 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