{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T08:05:00Z","timestamp":1789718700238,"version":"4.0.1"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objective<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Brucea javanica<\/jats:italic>\n                      oil emulsion (BJOE), a traditional Chinese medicine extract, has been demonstrated to possess antitumor activity in multiple cancers. However, its molecular mechanisms in breast cancer, particularly those involving circRNAs, remain unclear. This study investigates whether BJOE exerts its anti\u2010breast cancer effects by inducing autophagy and apoptosis through regulating hsa_circ_0001589\/G3BP1\/PAX6 signalling axis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>After BJOE treatment of MCF\u20107 cells, cell viability, apoptosis (Annexin V\u2010FITC\/PI staining) and autophagy (LC3\u2010II\/LC3\u2010I ratio, autophagosome formation) were assessed. qPCR identified hsa_circ_0001589 as a key circRNA downregulated BJOE. RNA immunoprecipitation (RIP) and Western blot confirmed the interaction between hsa_circ_0001589 and G3BP1 and subsequent PAX6 inhibition. This axis was verified via functional rescue experiments (siRNA knockdown\/overexpression).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      BJOE markedly inhibited MCF\u20107 cells\u00a0proliferation, induced apoptosis and activated autophagy in a dose\u2010dependent manner. Mechanistically, BJOE downregulated hsa_circ_0001589, thereby weakening the interaction between hsa_circ_0001589 and G3BP1 and reducing G3BP1\u2010mediated stabilisation of\n                      <jats:italic>PAX6<\/jats:italic>\n                      mRNA. Silencing hsa_circ_0001589 mimicked the effects of BJOE, whereas overexpression of hsa_circ_0001589 and G3BP1 attenuated BJOE\u2010induced autophagy and apoptosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>BJOE promotes autophagy and apoptosis through the hsa_circ_0001589\/G3BP1\/PAX6 axis in MCF\u20107 breast cancer models, offering a novel therapeutic strategy for breast cancer and providing a circRNA\u2010mediated mechanism for natural anticancer drug research.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1440-1681.70150","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T07:58:41Z","timestamp":1787903921000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Brucea javanica<\/i>\n                    Oil Emulsion Promotes Breast Cancer Cell Autophagy and Apoptosis by Regulating\n                    <scp>\n                      <i>PAX6<\/i>\n                      mRNA\n                    <\/scp>\n                    Stability Through the hsa_circ_0001589\/\n                    <scp>G3BP1<\/scp>\n                    Axis"],"prefix":"10.1111","volume":"53","author":[{"given":"Ke","family":"Li","sequence":"first","affiliation":[{"name":"Department of Cancer Center Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan City Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MeiXia","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cancer Center Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan City Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XueQiang","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Cancer 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