{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T08:04:50Z","timestamp":1789718690538,"version":"4.0.1"},"reference-count":19,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To analyse the immunological characteristics of low\u2010density neutrophils (LDNs) in patients with tuberculosis (TB).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study systematically characterized the immunological features of LDNs in active TB patients by comparing the proportion, phenotype and function of peripheral blood LDNs between healthy controls (HCs) and TB patients. Single\u2010cell RNA sequencing analysis and quantitative PCR validation were performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The proportion of LDNs was significantly increased in TB patients, exhibiting an enhanced effector phenotype but impaired migration and antibody\u2010dependent functions. The direct bactericidal activity of LDNs against\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Mycobacterium bovis<\/jats:italic>\n                      <\/jats:styled-content>\n                      BCG was compromised. Single\u2010cell sequencing analysis revealed significant upregulation of genes associated with ferroptosis and neutrophil extracellular trap (NETosis) formation pathways in TB patients' LDNs. Key ferroptosis\u2010related genes HMOX1 and ACSL4 were upregulated, while GPX4 and FSP1 were downregulated, indicating increased susceptibility to ferroptosis. Functional intervention experiments demonstrated that treatment with the ferroptosis inhibitor Ferrostatin\u20101 enhanced the bactericidal capacity of LDNs against BCG.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Ferroptosis and NETosis pathway activation in LDNs of TB patients leads to functional exhaustion and exacerbates immunopathology, providing new insights for immune intervention strategies in TB.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1440-1681.70155","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T09:31:12Z","timestamp":1789119072000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ferroptosis in Low\u2010Density Neutrophils of Tuberculosis Patients Suppresses Bactericidal Function"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7092-8256","authenticated-orcid":false,"given":"Li","family":"Jiaqi","sequence":"first","affiliation":[{"name":"Clinical Research Center of Tuberculosis Shanghai Pulmonary Hospital, Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Xuejiao","sequence":"additional","affiliation":[{"name":"Shanghai Institute of Infectious Diseases and Biosecurity Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Wei","sequence":"additional","affiliation":[{"name":"Clinical Research Center of Tuberculosis Shanghai Pulmonary Hospital, Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shen","family":"Hongbo","sequence":"additional","affiliation":[{"name":"Clinical Research Center of Tuberculosis Shanghai Pulmonary Hospital, Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985819833454"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1183\/16000617.0077-2017"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.16584.1"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.703846"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048939"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-04-713206"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12150"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-3701-4"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-99182-7"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-021-00993-3"},{"issue":"3","key":"e_1_2_12_12_1","first-page":"420","article-title":"Selective Destruction of Interleukin 23\u2010Induced Expansion of a Major Antigen\u2010Specific \u03b3\u03b4 T\u2010Cell Subset in Patients With Tuberculosis","volume":"215","author":"Shen H.","year":"2017","journal-title":"Journal of Infectious Diseases"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.669394"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201444635"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.739219"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.599641"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02761"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1701073"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.06.044"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101328"}],"container-title":["Clinical and 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