{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T03:39:44Z","timestamp":1792381184714,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960478"],"award-info":[{"award-number":["81960478"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82260549"],"award-info":[{"award-number":["82260549"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Ras\u2010related protein Rab\u201027A (RAB27A) is involved in tumour progression and immune regulation. This study investigated its role in triple\u2010negative breast cancer (TNBC) progression, immune escape and responsiveness to immune checkpoint inhibitors. RAB27A expression was analysed in TNBC tissues and public datasets. Gain\u2010 and loss\u2010of\u2010function experiments were performed in TNBC cells. Peripheral blood mononuclear cell (PBMC)\u2010based tumour coculture systems and xenograft mouse models were used to evaluate antitumor immunity and therapeutic responses. RAB27A was upregulated in TNBC and correlated with chemotherapy resistance, advanced T stage, high Ki\u201067 expression and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell infiltration. RAB27A overexpression activated PI3K\/Akt\/mTOR signalling and promoted tumour cell migration and invasion, whereas RAB27A silencing had the opposite effects. Combined RAB27A targeting and PI3K inhibition decreased PD\u2010L1 expression and cell viability while increasing HLA\u2010I expression and apoptosis; these effects were further enhanced by pembrolizumab. In PBMC coculture models, combination treatment promoted T\u2010cell proliferation and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell expansion while suppressing malignant phenotypes. In\u00a0vivo, RAB27A knockdown inhibited tumour growth, reduced PI3K\/Akt\/mTOR activation and PD\u2010L1 expression and increased HLA\u2010I expression. Targeting RAB27A enhances antitumor immunity and pembrolizumab sensitivity by blocking the PI3K\/Akt\/mTOR axis, representing a potential strategy to overcome immune resistance in TNBC.\n                  <\/jats:p>","DOI":"10.1111\/1440-1681.70159","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T11:30:22Z","timestamp":1790940622000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeting\n                    <scp>RAB27A<\/scp>\n                    Enhances Pembrolizumab Sensitivity and Alleviates Immune Escape via Inactivation of the\n                    <scp>PI3K<\/scp>\n                    \/Akt\/\n                    <scp>mTOR<\/scp>\n                    Pathway in Triple\u2010Negative Breast Cancer"],"prefix":"10.1111","volume":"53","author":[{"given":"Tong","family":"Sha","sequence":"first","affiliation":[{"name":"Department of Breast Thyroid Surgery, Xinjiang Medical University Affiliated Tumor Hospital The Clinical Medical Research Center of Breast and Thyroid Tumors in Xinjiang  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Breast Thyroid Surgery, Xinjiang Medical University Affiliated Tumor Hospital The Clinical Medical Research Center of Breast and Thyroid Tumors in Xinjiang  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Breast Thyroid Surgery, Xinjiang Medical University Affiliated Tumor Hospital The Clinical Medical Research Center of Breast and Thyroid Tumors in Xinjiang  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3684-7735","authenticated-orcid":false,"given":"Binlin","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Breast Thyroid Surgery, Xinjiang Medical University Affiliated Tumor Hospital The Clinical Medical Research Center of Breast and Thyroid Tumors in Xinjiang  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,2]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/CM9.0000000000003921"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.37349\/etat.2026.1002372"},{"issue":"5","key":"e_1_2_13_5_1","article-title":"Guidance for Canadian Breast Cancer Practice: National Consensus Recommendations for the Systemic Treatment of Patients With Triple Negative Breast Cancer in Both the Early and Metastatic Setting 2025","volume":"33","author":"Simmons C.","year":"2026","journal-title":"Current Oncology (Toronto, 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