{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T02:23:26Z","timestamp":1778207006292,"version":"3.51.4"},"reference-count":51,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,3,11]],"date-time":"2013-03-11T00:00:00Z","timestamp":1362960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2013,8]]},"abstract":"Summary<\/jats:title>\n \n Microbial reductive dechlorination of trichloroethene (\n TCE<\/jats:styled-content>\n ) in groundwater often results in the accumulation of dichloroethenes (\n DCE<\/jats:styled-content>\n s).\n \n D<\/jats:styled-content>\n ehalococcoides mccartyi\n <\/jats:italic>\n (\n \n D<\/jats:styled-content>\n hc\n <\/jats:italic>\n ) are the only known bacteria capable of dechlorination beyond\n DCE<\/jats:styled-content>\n to non\u2010toxic ethene. In this study, two newly isolated\n \n D<\/jats:styled-content>\n hc\n <\/jats:italic>\n strains (11a and 11a5) with dissimilar functional abilities are described. Strain 11a reductively dechlorinates\n TCE<\/jats:styled-content>\n , 1,1\u2010\n DCE<\/jats:styled-content>\n ,\n cis<\/jats:italic>\n \u2010\n DCE<\/jats:styled-content>\n ,\n trans<\/jats:italic>\n \u2010\n DCE<\/jats:styled-content>\n , and vinyl chloride (\n VC<\/jats:styled-content>\n ) to ethene, while strain 11a5 dechlorinates\n TCE<\/jats:styled-content>\n and all three\n DCE<\/jats:styled-content>\n isomers only to\n VC<\/jats:styled-content>\n . Each of these dechlorination reactions are coupled to growth by these strains. The\n VC<\/jats:styled-content>\n dechlorination rate of strain 11a occurs at a rate of 258\u2009nmol per min per mg of protein, about two times faster than previously reported stains. Strain 11a possesses the\n \n vcrA<\/jats:italic>\n <\/jats:styled-content>\n gene while strain 11a5 contains the\n \n tceA<\/jats:italic>\n <\/jats:styled-content>\n gene. Strains 11a and 11a5 share 100% 16\n S rRNA<\/jats:styled-content>\n gene sequence identity with previously sequenced\n \n D<\/jats:styled-content>\n hc\n <\/jats:italic>\n strains\n BAV1<\/jats:styled-content>\n and\n CBDB1<\/jats:styled-content>\n , placing it within the\n P<\/jats:styled-content>\n inellas subgroup, and 85.4% and 89.5% of all genes present in the\n CBDB1<\/jats:styled-content>\n and\n BAV1<\/jats:styled-content>\n genomes were detected in strains 11a and 11a5, respectively, using a custom\u2010designed microarray targeting four sequenced\n \n D<\/jats:styled-content>\n hc\n <\/jats:italic>\n strains. Genes that were not detected in strains 11a and 11a5 are mostly within the high plasticity regions or integrated elements of the sequenced strains. This study reports the functional description and comparative genomics of two additional\n \n D<\/jats:styled-content>\n hc\n <\/jats:italic>\n isolates and provides evidence that the observed functional incongruence between the activity and core genome phylogenies of\n \n D<\/jats:styled-content>\n hc\n <\/jats:italic>\n strains is likely driven by the horizontal transfer of key reductive dehalogenase\u2010encoding genes.\n <\/jats:p>","DOI":"10.1111\/1462-2920.12099","type":"journal-article","created":{"date-parts":[[2013,1,30]],"date-time":"2013-01-30T04:57:06Z","timestamp":1359521826000},"page":"2293-2305","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Isolation of two new\n \n D<\/scp>\n ehalococcoides mccartyi\n <\/i>\n strains with dissimilar dechlorination functions and their characterization by comparative genomics via microarray analysis"],"prefix":"10.1111","volume":"15","author":[{"given":"Patrick K. 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Lawrence Berkeley National Laboratory Berkeley CA 94720 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianzhong","family":"He","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering National University of Singapore Singapore 117576"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,3,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsec.2005.03.003"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01649-07"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/es062076m"},{"key":"e_1_2_6_5_1","volume-title":"Toxicological Profile for 1,2\u2010Dichloroethene","author":"ATSDR","year":"1996"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00926-08"},{"key":"e_1_2_6_7_1","volume-title":"Concise International Chemical Assessment Document 51","author":"Benson 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