{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:40:40Z","timestamp":1790145640273,"version":"4.0.1"},"reference-count":56,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,5,6]],"date-time":"2013-05-06T00:00:00Z","timestamp":1367798400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2013,7]]},"abstract":"Summary<\/jats:title>\n \n Phosphorus (\n P<\/jats:styled-content>\n ) availability drives niche differentiation in the most abundant phytoplankter in the oceans, the marine cyanobacterium\n \n P<\/jats:styled-content>\n rochlorococcus\n <\/jats:italic>\n . We compared the molecular response of\n \n P<\/jats:styled-content>\n rochlorococcus\n <\/jats:italic>\n strain\n MED4<\/jats:styled-content>\n to\n P<\/jats:styled-content>\n starvation in batch culture to\n P<\/jats:styled-content>\n \u2010limited growth in chemostat culture. We also identified an outer membrane porin,\n PMM0709<\/jats:styled-content>\n , which may allow transport of organic phosphorous compounds, rather than phosphate as previously suggested. The expression of three\n P<\/jats:styled-content>\n uptake genes,\n \n pstS<\/jats:italic>\n <\/jats:styled-content>\n , the high\u2010affinity phosphate\u2010binding component of the phosphate transporter,\n \n phoA<\/jats:italic>\n <\/jats:styled-content>\n , an alkaline phosphatase, and porin\n PMM0709<\/jats:styled-content>\n , were strongly upregulated (between 10\u2010 and 700\u2010fold) under both\n P<\/jats:styled-content>\n starvation and limitation.\n \n pstS<\/jats:italic>\n <\/jats:styled-content>\n exhibits high basal expression under\n P<\/jats:styled-content>\n \u2010replete conditions and is likely necessary for\n P<\/jats:styled-content>\n uptake regardless of\n P<\/jats:styled-content>\n availability. A\n P<\/jats:styled-content>\n \u2010stress regulatory gene,\n \n ptrA<\/jats:italic>\n <\/jats:styled-content>\n , was upregulated in response to both\n P<\/jats:styled-content>\n starvation and limitation although a second regulatory gene,\n \n phoB<\/jats:italic>\n <\/jats:styled-content>\n , was not. Elevated expression levels (>\u200910\u2010fold) of\n \n phoR<\/jats:italic>\n <\/jats:styled-content>\n , a\n P<\/jats:styled-content>\n \u2010sensing histidine kinase, were only observed under conditions of\n P<\/jats:styled-content>\n limitation. We suggest\n \n P<\/jats:styled-content>\n rochlorococcus\n <\/jats:italic>\n in\n P<\/jats:styled-content>\n \u2010limited systems are physiologically distinct from cells subjected to abrupt\n P<\/jats:styled-content>\n depletion. Detection of expression of both\n \n pstS<\/jats:italic>\n <\/jats:styled-content>\n and\n \n phoR<\/jats:italic>\n <\/jats:styled-content>\n in field populations will enable discernment of the present\n P<\/jats:styled-content>\n status of\n \n P<\/jats:styled-content>\n rochlorococcus\n <\/jats:italic>\n in the oligotrophic oceans.\n <\/jats:p>","DOI":"10.1111\/1462-2920.12129","type":"journal-article","created":{"date-parts":[[2013,4,9]],"date-time":"2013-04-09T01:25:14Z","timestamp":1365470714000},"page":"2129-2143","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Effects of phosphorus starvation versus limitation on the marine cyanobacterium\n \n P<\/scp>\n rochlorococcus\n <\/i>\n \u2005\n MED4 II<\/scp>\n : gene expression"],"prefix":"10.1111","volume":"15","author":[{"given":"Emily Nahas","family":"Reistetter","sequence":"first","affiliation":[{"name":"School of Oceanography Center for Environmental 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