{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T06:23:02Z","timestamp":1781158982137,"version":"3.54.1"},"reference-count":62,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,9,30]],"date-time":"2013-09-30T00:00:00Z","timestamp":1380499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Characterizing both growth and abundance is important in understanding the role of bacterial communities in biogeochemical cycling of global oceans. However, these two quantities are seldom measured together for specific bacterial clades. Our goal was to examine growth and abundance of three gammaproteobacterial subgroups, including\n                    <jats:styled-content style=\"fixed-case\">SAR<\/jats:styled-content>\n                    86, at the single\u2010cell level by microautoradiography combined with fluorescence in situ hybridization (\n                    <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>\n                    ) in coastal waters of the west\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    ntarctic\n                    <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>\n                    eninsula region during two austral summers and one austral fall. We found that the\n                    <jats:styled-content style=\"fixed-case\">SAR<\/jats:styled-content>\n                    86 clade was less abundant and grew more slowly than two related gammaproteobacterial clades,\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    nt4\n                    <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>\n                    3 and\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    rctic96\n                    <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                    \u201016. Over 60% of\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    nt4\n                    <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>\n                    3 and\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    rctic96\n                    <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                    \u201016 cells incorporated leucine, while only 25% of\n                    <jats:styled-content style=\"fixed-case\">SAR<\/jats:styled-content>\n                    86 cells were active in both summer and fall. We also explored using the size of the\n                    <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>\n                    image as another measure of single\u2010cell activity. There was a linear relationship between\n                    <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>\n                    cell size and incorporation of leucine for all bacteria,\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    nt4\n                    <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>\n                    3 and\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    rctic96\n                    <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                    \u201016, but not for\n                    <jats:styled-content style=\"fixed-case\">SAR<\/jats:styled-content>\n                    86.\n                    <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>\n                    sizes of\n                    <jats:styled-content style=\"fixed-case\">SAR<\/jats:styled-content>\n                    86 cells were at least threefold smaller than cells in the other clades. Our results suggest slow growth of\n                    <jats:styled-content style=\"fixed-case\">SAR<\/jats:styled-content>\n                    86 in the perennially cold waters of the west Antarctic Peninsula.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.12258","type":"journal-article","created":{"date-parts":[[2013,8,30]],"date-time":"2013-08-30T06:05:09Z","timestamp":1377842709000},"page":"1513-1523","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Growth activity of gammaproteobacterial subgroups in waters off the west\n                    <scp>A<\/scp>\n                    ntarctic\n                    <scp>P<\/scp>\n                    eninsula in summer and fall"],"prefix":"10.1111","volume":"16","author":[{"given":"Mrinalini P.","family":"Nikrad","sequence":"first","affiliation":[{"name":"School of Marine Science and Policy University of Delaware  700 Pilottown Road Lewes DE 19958 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew T.","family":"Cottrell","sequence":"additional","affiliation":[{"name":"School of Marine Science and Policy University of Delaware  700 Pilottown Road Lewes DE 19958 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L.","family":"Kirchman","sequence":"additional","affiliation":[{"name":"School of Marine Science and Policy University of Delaware  700 Pilottown Road Lewes DE 19958 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,9,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3354\/ame045237"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2006.01082.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2010.69"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00597-06"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2008.01674.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1888"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.2.762-770.1990"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.54.1.105-109.1988"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.2.505-518.2002"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.9.3346-3351.1998"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.3354\/ame01335"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.4.1692-1697.2000"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2003.48.1.0168"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2466341"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0967-0645(01)00086-8"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0967-0645(95)00022-I"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dsr2.2006.02.009"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2006.1955"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmarsys.2012.03.003"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.189"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.7.3044-3051.2000"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.3354\/ame01145"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.11.4433-4438.1998"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.21.6780-6788.1992"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00118"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmarsys.2008.09.002"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02601.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1208160109"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470281840.ch8"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1114057"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00170244"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.2.1532-1541.2006"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.31"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12237-011-9386-6"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.3354\/ame01317"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.1999.tb00653.x"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0580-9517(01)30047-8"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2007.52.2.0495"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2115"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.1994.39.4.0869"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.6.2979-2986.2005"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.3354\/ame047045"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2006.1944"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dsr2.2010.11.021"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07130-11"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.9.4077-4083.2001"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.6.3094-3101.2002"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.2.661-667.2002"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.59.5.1354-1360.1993"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)01341-0"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00917"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.01929.x"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00763-06"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2010.55.6.2526"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10872-012-0114-z"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000808"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2012.02720.x"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.10.2893-2899.2000"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(11)80396-6"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990140205"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.3354\/ame01443"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11921"}],"container-title":["Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1462-2920.12258","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1462-2920.12258","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T05:14:56Z","timestamp":1761887696000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1462-2920.12258"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,30]]},"references-count":62,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1111\/1462-2920.12258"],"URL":"https:\/\/doi.org\/10.1111\/1462-2920.12258","archive":["Portico"],"relation":{},"ISSN":["1462-2912","1462-2920"],"issn-type":[{"value":"1462-2912","type":"print"},{"value":"1462-2920","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,30]]},"assertion":[{"value":"2013-04-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-09-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}