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The purpose of this study was to determine whether shifts between motile and sessile life in the globally abundant\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\n                      oseobacter\n                    <\/jats:italic>\n                    clade species\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\n                      uegeria mobilis\n                    <\/jats:italic>\n                    are associated with intracellular concentrations of the signal compound cyclic dimeric guanosinmonophosphate (c\u2010di\u2010\n                    <jats:styled-content style=\"fixed-case\">GMP<\/jats:styled-content>\n                    ), which in bacteria regulates transitions between motile and sessile life stages. Genes for diguanylate cyclases and phosphodiesterases, which are involved in c\u2010di\u2010\n                    <jats:styled-content style=\"fixed-case\">GMP<\/jats:styled-content>\n                    signalling, were found in the genome of\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\n                      .\u2009mobilis\n                    <\/jats:italic>\n                    strain\n                    <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>\n                    1926. Ion pair chromatography\u2010tandem mass spectrometry revealed 20\u2010fold higher c\u2010di\u2010\n                    <jats:styled-content style=\"fixed-case\">GMP<\/jats:styled-content>\n                    concentrations per cell in biofilm\u2010containing cultures than in planktonic cells. An introduced diguanylate cyclase gene increased c\u2010di\u2010\n                    <jats:styled-content style=\"fixed-case\">GMP<\/jats:styled-content>\n                    and enhanced biofilm formation and production of the potent antibiotic tropodithietic acid (\n                    <jats:styled-content style=\"fixed-case\">TDA<\/jats:styled-content>\n                    ). An introduced phosphodiesterase gene decreased c\u2010di\u2010\n                    <jats:styled-content style=\"fixed-case\">GMP<\/jats:styled-content>\n                    and reduced biofilm formation and\n                    <jats:styled-content style=\"fixed-case\">TDA<\/jats:styled-content>\n                    production.\n                    <jats:italic>tda<\/jats:italic>\n                    <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\n                    , a key gene for\n                    <jats:styled-content style=\"fixed-case\">TDA<\/jats:styled-content>\n                    biosynthesis, was expressed only in attached or biofilm\u2010forming cells, and expression was induced immediately after initial attachment. In conclusion, c\u2010di\u2010\n                    <jats:styled-content style=\"fixed-case\">GMP<\/jats:styled-content>\n                    signalling controls biofilm formation and biofilm\u2010associated traits in\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\n                      .\u2009mobilis\n                    <\/jats:italic>\n                    and, as suggested by presence of\n                    <jats:styled-content style=\"fixed-case\">GGDEF<\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">EAL<\/jats:styled-content>\n                    domain protein genes, also in other\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\n                      oseobacter\n                    <\/jats:italic>\n                    clade species.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.12265","type":"journal-article","created":{"date-parts":[[2013,9,4]],"date-time":"2013-09-04T13:03:31Z","timestamp":1378299811000},"page":"1252-1266","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Biofilm formation and antibiotic production in\n                    <i>\n                      <scp>R<\/scp>\n                      uegeria mobilis\n                    <\/i>\n                    are influenced by intracellular concentrations of cyclic dimeric guanosinmonophosphate"],"prefix":"10.1111","volume":"16","author":[{"given":"Paul W.","family":"D'Alvise","sequence":"first","affiliation":[{"name":"Department of Systems Biology Technical University of Denmark  S\u00f8ltofts Plads Bldg. 221 DK\u20102800 Kgs. Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivera","family":"Magdenoska","sequence":"additional","affiliation":[{"name":"Department of Systems Biology Technical University of Denmark  S\u00f8ltofts Plads Bldg. 221 DK\u20102800 Kgs. Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jette","family":"Melchiorsen","sequence":"additional","affiliation":[{"name":"Department of Systems Biology Technical University of Denmark  S\u00f8ltofts Plads Bldg. 221 DK\u20102800 Kgs. Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristian F.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Systems Biology Technical University of Denmark  S\u00f8ltofts Plads Bldg. 221 DK\u20102800 Kgs. 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